Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	147,516	C→T	R183R (CGC→CGT)	rpnC →	recombination‑promoting nuclease RpnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	147,516	0	C	T	100.0%	126.9 / NA	35	R183R (CGC→CGT)	rpnC	recombination‑promoting nuclease RpnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/17);  total (18/17)

ACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCAGTT  >  NC_000913/147442‑147590                                                                           |                                                                           aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAgcgc                                                                      <  1:32375/81‑1 (MQ=255)     tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACt                                                                  >  2:32441/1‑81 (MQ=255)        cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTaa                                                               >  2:32430/1‑81 (MQ=255)           cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGt                                                            <  1:32376/81‑1 (MQ=255)            ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGtt                                                           <  2:32377/81‑1 (MQ=255)             gATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTa                                                          >  1:32425/1‑81 (MQ=255)                gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTg                                                       >  1:32418/1‑81 (MQ=255)                 aCGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGc                                                      >  1:32435/1‑81 (MQ=255)                   gAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCtt                                                    <  1:32379/81‑1 (MQ=255)                   gAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCtt                                                    <  2:32378/81‑1 (MQ=255)                         aTGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCa                                               <  2:32380/80‑1 (MQ=255)                         aTGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCa                                               <  2:32381/80‑1 (MQ=255)                                aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa                                       >  1:32437/1‑81 (MQ=255)                                 tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc                                      >  2:32434/1‑81 (MQ=255)                                    gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt                                   >  2:32429/1‑81 (MQ=255)                                    gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt                                   >  2:32423/1‑81 (MQ=255)                                      ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                                 >  1:32420/1‑81 (MQ=255)                                      ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                                 <  1:32382/81‑1 (MQ=255)                                        aTTGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                               <  1:32383/81‑1 (MQ=255)                                         ttGCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcg                              <  2:32384/81‑1 (MQ=255)                                           gCGATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgac                            >  1:32446/1‑81 (MQ=255)                                             gATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacg                           >  2:32432/1‑80 (MQ=255)                                             gATTCTCGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacg                           >  1:32428/1‑80 (MQ=255)                                                   cGAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa                    >  2:32440/1‑81 (MQ=255)                                                    gAACTACTGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa                    >  2:32449/1‑80 (MQ=255)                                                          ctGCAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGTGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGc             <  1:32385/81‑1 (MQ=255)                                                             cAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg           <  1:32386/80‑1 (MQ=255)                                                             cAAAAACATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg           <  2:32387/80‑1 (MQ=255)                                                                aaaCATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGt       <  2:32389/81‑1 (MQ=255)                                                                aaaCATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGt       <  1:32388/81‑1 (MQ=255)                                                                   cATATTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCag    <  2:32390/81‑1 (MQ=255)                                                                    atatTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCagt   >  1:32436/1‑81 (MQ=255)                                                                     tatTCGTCAGCGCGACTTAATGTTATTGCTTGCGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCagt   >  2:32448/1‑80 (MQ=255)                                                                     tatTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCagtt  <  1:32391/81‑1 (MQ=255)                                                                      atTCGTCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCagt   >  2:32453/1‑79 (MQ=255)                                                                           |                                                                           ACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCAGTT  >  NC_000913/147442‑147590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑