Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,198,874	A→G	E60E (GAA→GAG)	lit →	e14 prophage; cell death peptidase Lit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,198,874	0	A	G	100.0%	45.2 / NA	14	E60E (GAA→GAG)	lit	e14 prophage; cell death peptidase Lit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/8);  total (6/8)

TTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAACAGTAGCTTCACTTGAATATATTTGGGCATTTTCCAATTTCTTTTGGGTTTTTACGCAAG  >  NC_000913/1198801‑1198944                                                                          |                                                                       ttATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTc                                                                 >  2:215423/1‑81 (MQ=255)   ataATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGATTGAGAGTAATAATTCACATGAGATAGTTCt                                                                >  2:215426/1‑80 (MQ=255)      aTTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTCTAAc                                                             <  1:215404/80‑1 (MQ=255)      aTTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTCTAAc                                                             <  1:215405/80‑1 (MQ=255)          cAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTCTAACAGTAg                                                        >  1:215419/1‑81 (MQ=255)                            gTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTCTAACAGTAGCTTCACTTGAATATAttt                                      >  1:215424/1‑81 (MQ=255)                            gTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTCTAACAGTAGCTTCACTTGAATATAttt                                      >  2:215428/1‑81 (MQ=255)                                  atatCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTCTAACAGTAGCTTCACTTGAATATATTTGGGCAt                                >  2:215425/1‑81 (MQ=255)                                       cGAGTCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTCTAACAGTAGCTTCACTTGAATATATTTGGGCATTTTc                            <  1:215406/80‑1 (MQ=255)                                          gTCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTCTAACAGTAGCTTCACTTGAATATATTTGGGCATTTTCCAAt                        <  1:215407/81‑1 (MQ=255)                                           tCAGGAAGAATGAGAGTAATAATTCACATGAGATAGTTCTAACAGTAGCTTCACTTGAATATATTTGGGCATTTTCCAAtt                       <  2:215408/81‑1 (MQ=255)                                                aagaaTGAGAGTAATAATTCACATGAGATAGTTCTAACAGTAGCTTCACTTGAATATATTTGGGCATTTTCCAATTTCttt                  <  2:215409/81‑1 (MQ=255)                                                           taataaTTCACATGAGATAGTTCTAACAGTAGCTTCACTTGAATATATTTGGGCATTTTCCAATTTCTTTTGGGTTTTTAc       <  1:215410/81‑1 (MQ=255)                                                                atTCACATGAGATAGTTCTAACAGTAGCTTCACTTGAATATATTTGGGCATTTTCCAATTTCTTTTGGGTTTTTACGCAag  <  2:215411/81‑1 (MQ=255)                                                                          |                                                                       TTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAACAGTAGCTTCACTTGAATATATTTGGGCATTTTCCAATTTCTTTTGGGTTTTTACGCAAG  >  NC_000913/1198801‑1198944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑