Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,217,089	C→T	intergenic (+93/‑239)	ymgC → / → pdeG	protein YmgC/putative c‑di‑GMP phosphodiesterase PdeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,217,089	0	C	T	100.0%	50.8 / NA	15	intergenic (+93/‑239)	ymgC/pdeG	protein YmgC/putative c‑di‑GMP phosphodiesterase PdeG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/9);  total (6/9)

GAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCT  >  NC_000913/1217015‑1217151                                                                           |                                                               gAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATc                                                          <  2:217639/81‑1 (MQ=255)       aCATCAGCTGTATCACCATGCTGATGCAAAGTGGGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGgtgt                                                    <  1:217640/81‑1 (MQ=255)        cATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTc                                                   <  1:217641/81‑1 (MQ=255)        cATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTc                                                   <  2:217642/81‑1 (MQ=255)             gCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGaa                                              >  1:217654/1‑81 (MQ=255)                     aCCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGt                                      >  2:217659/1‑81 (MQ=255)                           cTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGTTCTCGc                                <  1:217643/81‑1 (MQ=255)                           cTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGTTCTCGc                                <  1:217644/81‑1 (MQ=255)                             gATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGTTCTCGctc                              >  1:217653/1‑81 (MQ=255)                                      tGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCtt                     <  2:217645/81‑1 (MQ=255)                                       gAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCtt                     >  1:217656/1‑80 (MQ=255)                                        aGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGa                   <  2:217646/81‑1 (MQ=255)                                             ttCAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCt              >  2:217661/1‑81 (MQ=255)                                               cAGGCGTTATGCTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGa            >  2:217658/1‑81 (MQ=255)                                                          cTTTCTTATTATGTCCGTAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCt  <  1:217647/80‑1 (MQ=255)                                                                           |                                                               GAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCT  >  NC_000913/1217015‑1217151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑