Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,016	T→C	Q313R (CAA→CGA)	yadC ←	fimbrial tip‑adhesin YadC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,016	0	T	C	100.0%	70.2 / NA	21	Q313R (CAA→CGA)	yadC	fimbrial tip‑adhesin YadC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/10);  total (11/10)

TAAACCTTCAATGAGTACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGC  >  NC_000913/149940‑150081                                                                             |                                                                  tAAACCTTCAATGAGTACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAg                                                                >  1:32929/1‑80 (MQ=255)  aaaCCTTCAATGAGTACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTa                                                              >  1:32939/1‑81 (MQ=255)    aCCTTCAATGAGTACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTAcc                                                            <  2:32903/81‑1 (MQ=255)     ccTTCAATGAGTACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCt                                                           <  2:32904/81‑1 (MQ=255)        tCAATGAGTACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACt                                                        >  2:32926/1‑81 (MQ=255)         cAATGAGTACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACATAc                                                         <  1:32905/79‑1 (MQ=255)             gAGTACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTc                                                    >  2:32941/1‑80 (MQ=255)                tACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGt                                                <  1:32906/81‑1 (MQ=255)                 aCGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGt                                                >  1:32935/1‑80 (MQ=255)                 aCGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGt                                                >  2:32924/1‑80 (MQ=255)                 aCGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGt                                                >  2:32925/1‑80 (MQ=255)                  cGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGTg                                               <  1:32907/80‑1 (MQ=255)                     cAACGCCTTTGGCGGCCGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGTGAc                                             >  2:32934/1‑79 (MQ=255)                      aaCGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGTGACGAg                                          >  1:32940/1‑81 (MQ=255)                                  ggcAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAat                              <  2:32908/81‑1 (MQ=255)                                     aGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAatat                            >  1:32936/1‑80 (MQ=255)                                              aGTAAGCGTATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACg                  <  1:32909/81‑1 (MQ=255)                                                      tATTACCAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAAt          <  2:32910/81‑1 (MQ=255)                                                           ccAAGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTg      <  2:32911/80‑1 (MQ=255)                                                             aaGCAGTTGTGTGTTTCGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAg   <  2:32912/81‑1 (MQ=255)                                                                 aGTTGTGTGTTTCGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGc  >  1:32944/1‑78 (MQ=255)                                                                             |                                                                  TAAACCTTCAATGAGTACGCCAACGCCTTTGGCGGCAGTGCTTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGC  >  NC_000913/149940‑150081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑