Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,163	A→G	I2V (ATT→GTT)	ycgY →	uncharacterized protein YcgY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,163	0	A	G	100.0%	29.0 / NA	10	I2V (ATT→GTT)	ycgY	uncharacterized protein YcgY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/5);  total (5/5)

CGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCT  >  NC_000913/1245087‑1245237                                                                             |                                                                           cGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTc                                                                         <  1:221296/80‑1 (MQ=255)            aaaGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAaataaata                                                             >  1:221313/1‑81 (MQ=255)                    ttCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGAc                                                     <  2:221297/81‑1 (MQ=255)                       ccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAg                                                   <  1:221298/80‑1 (MQ=255)                                  aacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAgg                                       >  2:221317/1‑81 (MQ=255)                                                  aaTTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCg                       >  2:221322/1‑81 (MQ=255)                                                             cGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGc            >  1:221315/1‑81 (MQ=255)                                                                  tttttGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGttt       <  2:221299/81‑1 (MQ=255)                                                                      tGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGc   >  2:221320/1‑81 (MQ=255)                                                                        gtgtGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCt  <  1:221300/80‑1 (MQ=255)                                                                             |                                                                           CGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCT  >  NC_000913/1245087‑1245237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑