Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,259,230	T→C	N525S (AAC→AGC)	dauA ←	aerobic C4‑dicarboxylate transporter DauA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,259,230	0	T	C	100.0%	87.9 / NA	26	N525S (AAC→AGC)	dauA	aerobic C4‑dicarboxylate transporter DauA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

GAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATCAAGACCACCAGCATCAAG  >  NC_000913/1259160‑1259306                                                                       |                                                                             gaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACAcgcg                                                                    >  2:223193/1‑81 (MQ=255) gaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACAcgcg                                                                    <  2:223159/81‑1 (MQ=255) gaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACAcgcg                                                                    <  2:223158/81‑1 (MQ=255)         ggCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTca                                                             >  1:223183/1‑80 (MQ=255)           cGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACAt                                                          >  2:223198/1‑81 (MQ=255)            gTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATc                                                         <  1:223160/81‑1 (MQ=255)                 gggATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCg                                                    >  2:223184/1‑81 (MQ=255)                    aTCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGc                                                 <  1:223161/81‑1 (MQ=255)                       ggTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAg                                               <  1:223162/80‑1 (MQ=255)                       ggTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGa                                              >  1:223190/1‑81 (MQ=255)                       ggTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGa                                              >  1:223194/1‑81 (MQ=255)                           gAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCt                                          >  2:223202/1‑81 (MQ=255)                             aTGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTc                                        <  1:223164/81‑1 (MQ=255)                             aTGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTc                                        <  1:223163/81‑1 (MQ=255)                              tGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTca                                       <  1:223165/81‑1 (MQ=255)                                      cgAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGc                               <  2:223166/81‑1 (MQ=255)                                       gAGCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCt                              >  2:223187/1‑81 (MQ=255)                                         gCCATAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTg                             >  2:223191/1‑80 (MQ=255)                                            aTAGTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAAc                         >  2:223201/1‑81 (MQ=255)                                               gTGCGCAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGc                       <  2:223167/80‑1 (MQ=255)                                                 gcgcAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCAt                     >  2:223199/1‑80 (MQ=255)                                                  cgcAGTGGCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATc                    <  1:223168/80‑1 (MQ=255)                                                        ggCTGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATCAAGacca             >  1:223188/1‑81 (MQ=255)                                                           tGGAATTCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATCAAGaccac            >  2:223200/1‑79 (MQ=255)                                                                ttCCACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATCAAGACCACCAGCAt      >  2:223208/1‑80 (MQ=255)                                                                   cACGCTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATCAAGACCACCAGCATCAAg  <  1:223169/81‑1 (MQ=255)                                                                       |                                                                             GAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATCAAGACCACCAGCATCAAG  >  NC_000913/1259160‑1259306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑