Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,276,549	T→A	D357V (GAT→GTT)	narX ←	sensory histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,276,549	0	T	A	100.0%	81.6 / NA	23	D357V (GAT→GTT)	narX	sensory histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/10);  total (13/10)

GCTGACGTTCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGATCATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTACTGCGTATGAGAGTCAGCCAGCCGC  >  NC_000913/1276472‑1276623                                                                              |                                                                           gCTGACGTTCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACAt                                                                         >  1:225676/1‑81 (MQ=255)    gACGTTCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGc                                                                      >  2:225675/1‑81 (MQ=255)     aCGTTCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGc                                                                      >  1:225683/1‑80 (MQ=255)     aCGTTCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCt                                                                     <  1:225648/81‑1 (MQ=255)        ttCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAg                                                                  <  1:225649/81‑1 (MQ=255)         tCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCt                                                                     >  1:225680/1‑77 (MQ=255)              aTGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGAc                                                             <  1:225650/80‑1 (MQ=255)                       cagcgccagcgTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCgg                                                   >  1:225685/1‑81 (MQ=255)                          cgccagcgTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAg                                                <  1:225651/81‑1 (MQ=255)                           gccagcgTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAgg                                               <  1:225652/81‑1 (MQ=255)                             cagcgTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGt                                             <  1:225653/81‑1 (MQ=255)                             cagcgTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGt                                             >  1:225677/1‑81 (MQ=255)                                    gCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAg                                       >  2:225688/1‑80 (MQ=255)                                            ttgttCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATAcc                              <  1:225654/81‑1 (MQ=255)                                                tCAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTAc                          >  1:225678/1‑81 (MQ=255)                                                 cAACCAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTAc                          <  2:225655/80‑1 (MQ=255)                                                    ccAGGGTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTACTGCg                      >  1:225692/1‑81 (MQ=255)                                                        ggTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTACTGCGTATg                  <  2:225656/81‑1 (MQ=255)                                                         gTATCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTACTGCGTAt                   >  1:225690/1‑79 (MQ=255)                                                           aTCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTACTGCGTATga                 >  2:225679/1‑79 (MQ=255)                                                            tCCACCAGTTGTTGTTGAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTACTGCGTATGagag              >  1:225684/1‑81 (MQ=255)                                                                       ttgttgAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTACTGCGTATGAGAGTCagccagcc    <  1:225657/80‑1 (MQ=255)                                                                         gttgAACATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTACTGCGTATGAGAGTCAGCCAgccgc  >  2:225686/1‑80 (MQ=255)                                                                              |                                                                           GCTGACGTTCCTGATGGCGATCCAGCGCCAGCGTGGCGGTGAGTTGTTCAACCAGGGTATCCACCAGTTGTTGTTGATCATGGCTAAGATGACGCCCCTGCGGCAGGGTCGCCAGCAAAATACCGTACTGCGTATGAGAGTCAGCCAGCCGC  >  NC_000913/1276472‑1276623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑