Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,277,223	G→C	A132A (GCC→GCG)	narX ←	sensory histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,277,223	0	G	C	100.0%	111.0 / NA	32	A132A (GCC→GCG)	narX	sensory histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/17);  total (15/17)

CGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTAC  >  NC_000913/1277149‑1277299                                                                           |                                                                             cGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGcaac                                                                         >  1:225767/1‑80 (MQ=255)  gATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGcaaca                                                                        >  1:225786/1‑80 (MQ=255)     ggACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACt                                                                    >  2:225780/1‑81 (MQ=255)        ccAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGc                                                                 <  1:225739/81‑1 (MQ=255)        ccAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGc                                                                 >  1:225771/1‑81 (MQ=255)          aGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTg                                                               <  2:225740/81‑1 (MQ=255)              ccACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACAt                                                           <  1:225741/81‑1 (MQ=255)                aCTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATcc                                                         <  2:225742/81‑1 (MQ=255)                   gTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCt                                                      >  2:225768/1‑81 (MQ=255)                    tctcGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGc                                                       <  1:225743/79‑1 (MQ=255)                    tctcGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCt                                                      <  2:225744/80‑1 (MQ=255)                     ctcGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGa                                                    >  2:225769/1‑81 (MQ=255)                        gATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGacac                                                 >  1:225776/1‑81 (MQ=255)                        gATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGacac                                                 <  2:225745/81‑1 (MQ=255)                         aTGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACAcc                                                <  2:225746/81‑1 (MQ=255)                          tGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCg                                               >  2:225778/1‑81 (MQ=255)                            cgcATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGtt                                             <  1:225747/81‑1 (MQ=255)                                tttCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTtctc                                         <  2:225748/81‑1 (MQ=255)                                   ccGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGt                                      >  2:225770/1‑81 (MQ=255)                                    cGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGt                                      <  2:225749/80‑1 (MQ=255)                                      tggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGtttt                                   <  1:225751/81‑1 (MQ=255)                                      tggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGtttt                                   <  2:225750/81‑1 (MQ=255)                                      tggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGtttt                                   >  1:225774/1‑81 (MQ=255)                                        gtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTtgt                                 >  1:225785/1‑81 (MQ=255)                                                aaaaCCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCa                          <  1:225752/80‑1 (MQ=255)                                                   aCCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCATGCATCAg                      <  2:225753/81‑1 (MQ=255)                                                      aGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAgcg                    >  1:225783/1‑80 (MQ=255)                                                        aTACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAg                 >  2:225779/1‑81 (MQ=255)                                                                tGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGt         >  1:225773/1‑81 (MQ=255)                                                                  aTCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCGCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTc       <  2:225754/81‑1 (MQ=255)                                                                    cAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCAt     >  1:225795/1‑81 (MQ=255)                                                                       gCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTAc  <  2:225755/81‑1 (MQ=255)                                                                           |                                                                             CGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTAC  >  NC_000913/1277149‑1277299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑