Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,296,554	C→T	S523N (AGC→AAC)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,296,554	0	C	T	100.0%	74.5 / NA	22	S523N (AGC→AAC)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/10);  total (12/10)

ATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACGGAAGTGATCT  >  NC_000913/1296476‑1296629                                                                               |                                                                            aTCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTc                                                                           <  2:228423/81‑1 (MQ=255)    ggggAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAgg                                                                        >  2:228443/1‑81 (MQ=255)         accaccaccCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAgggtcgg                                                                   <  2:228424/81‑1 (MQ=255)                 ccAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCtt                                                           <  2:228425/81‑1 (MQ=255)                  cAGCGCGATAATCACGTCTGGTTTGAAGGAGATTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTc                                                          <  2:228426/81‑1 (MQ=255)                        gATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTc                                                    <  1:228427/81‑1 (MQ=255)                        gATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTc                                                    <  2:228428/81‑1 (MQ=255)                         aTAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCg                                                   <  1:228429/81‑1 (MQ=255)                         aTAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCg                                                   <  2:228431/81‑1 (MQ=255)                         aTAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCg                                                   <  2:228430/81‑1 (MQ=255)                              cACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCgaag                                                >  2:228456/1‑79 (MQ=255)                              cACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCgaag                                                >  2:228460/1‑79 (MQ=255)                               aCGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCgaag                                                >  2:228446/1‑78 (MQ=255)                                 gTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGAc                                           >  2:228459/1‑81 (MQ=255)                                       tttGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGt                                     >  1:228453/1‑81 (MQ=255)                                        ttGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGtt                                    >  2:228449/1‑81 (MQ=255)                                              gAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACg                              >  2:228451/1‑81 (MQ=255)                                                         ttCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCttt                   >  2:228450/1‑81 (MQ=255)                                                           cTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCttt                   >  2:228448/1‑79 (MQ=255)                                                                    tACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACGGaa         <  1:228432/80‑1 (MQ=255)                                                                      cgaacgaTGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACGGAAGt       >  1:228463/1‑80 (MQ=255)                                                                           gaTGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACGGAAGtgatct  >  2:228447/1‑80 (MQ=255)                                                                               |                                                                            ATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACGGAAGTGATCT  >  NC_000913/1296476‑1296629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑