Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,299,534	T→C	F2L (TTT→CTT)	insH21 →	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,299,534	0	T	C	100.0%	26.3 / NA	9	F2L (TTT→CTT)	insH21	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/2);  total (7/2)

TTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCAT  >  NC_000913/1299461‑1299568                                                                          |                                   ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCAt                             <  1:228798/81‑1 (MQ=255)       aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAg                       >  1:228814/1‑81 (MQ=255)          ccACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCa                    >  2:228831/1‑81 (MQ=255)                 cTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGaa               >  1:228819/1‑79 (MQ=255)                     ggTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGt         >  2:228825/1‑81 (MQ=255)                        agagGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtca      >  1:228829/1‑81 (MQ=255)                           ggAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtcatca   >  2:228823/1‑81 (MQ=255)                           ggAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtcatca   >  2:228824/1‑81 (MQ=255)                            gAAAGTGCTAAGGAAGGTGCGAACAAGTCCCAGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTTcatcat  <  2:228799/81‑1 (MQ=255)                                                                          |                                   TTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCAT  >  NC_000913/1299461‑1299568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑