Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,004	C→A	V214V (GTG→GTT)	clsA ←	cardiolipin synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,004	0	C	A	100.0%	65.4 / NA	19	V214V (GTG→GTT)	clsA	cardiolipin synthase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/13);  total (6/13)

CTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTG  >  NC_000913/1307930‑1308079                                                                           |                                                                            ctgcCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACg                                                                        >  1:229972/1‑80 (MQ=255)    ccGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCAt                                                                     <  1:229942/80‑1 (MQ=255)       gtgtACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGa                                                                 <  1:229943/81‑1 (MQ=255)               gATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCtt                                                         >  2:229973/1‑81 (MQ=255)                 tGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTa                                                       <  2:229944/81‑1 (MQ=255)                   tAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACCCATCAGATTGACCTTTAAg                                                     >  2:229975/1‑81 (MQ=255)                         tCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTc                                                <  1:229945/80‑1 (MQ=255)                            atcatgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGAcc                                            <  1:229946/81‑1 (MQ=255)                               atgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACt                                         <  2:229947/81‑1 (MQ=255)                                        ttGCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCg                                >  2:229974/1‑81 (MQ=255)                                          gCGATGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATAcc                                 <  2:229948/78‑1 (MQ=255)                                          gCGATGTTCGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATAcc                                 <  1:229949/78‑1 (MQ=255)                                             aTGTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCAtt                           <  1:229950/81‑1 (MQ=255)                                               gTTGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTAc                         >  1:229977/1‑81 (MQ=255)                                                ttGGCGCAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTAc                         <  2:229951/80‑1 (MQ=255)                                                     gcAGGTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTa                   <  2:229952/81‑1 (MQ=255)                                                         gTCCATACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTc               >  2:229983/1‑81 (MQ=255)                                                              tACGGCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGcc          <  1:229953/81‑1 (MQ=255)                                                                 ggCGTAAAAAAACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCA‑TACGCATTAGCTCGGGCCACgg      <  1:229954/81‑1 (MQ=255)                                                                      aaaaaaACACGCATCAGATTGACCCTTAAGGCTTCGACCACTTCAATACTGGCATTACGCATTAGCTCGGGCCACGGGCTg  >  2:229976/1‑81 (MQ=255)                                                                           |                                                                            CTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTG  >  NC_000913/1307930‑1308079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑