Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,572	A→G	L25S (TTA→TCA)	clsA ←	cardiolipin synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,572	0	A	G	100.0%	48.0 / NA	15	L25S (TTA→TCA)	clsA	cardiolipin synthase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/9);  total (6/9)

CGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCAT  >  NC_000913/1308495‑1308645                                                                              |                                                                          cGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAg                                                                        >  2:230033/1‑81 (MQ=255)          gCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTg                                                               >  2:230036/1‑81 (MQ=255)            aGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCa                                                             <  2:230014/81‑1 (MQ=255)              aaTGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAAt                                                           >  1:230038/1‑81 (MQ=255)                   aaaTAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGc                                                       >  2:230037/1‑80 (MQ=255)                           aaCAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTa                                               <  1:230015/80‑1 (MQ=255)                             cAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATcc                                            <  1:230016/81‑1 (MQ=255)                              aGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATccc                                           <  2:230017/81‑1 (MQ=255)                                          gcgGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGccaac                               >  2:230031/1‑81 (MQ=255)                                           cgGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGccaacc                              <  2:230018/81‑1 (MQ=255)                                                    cTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCa                     <  1:230019/81‑1 (MQ=255)                                                       cgcgTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAAc                   <  1:230020/80‑1 (MQ=255)                                                        gcgTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGt                 <  1:230021/81‑1 (MQ=255)                                                                tCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACgg         >  2:230043/1‑81 (MQ=255)                                                                       aaTGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCAt  <  2:230022/81‑1 (MQ=255)                                                                              |                                                                          CGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCAT  >  NC_000913/1308495‑1308645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑