Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,313,464	A→G	V67A (GTT→GCT)	yciC ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,313,464	0	A	G	100.0%	74.1 / NA	22	V67A (GTT→GCT)	yciC	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/8);  total (14/8)

GATTAATCCTGAAAAAGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTG  >  NC_000913/1313391‑1313539                                                                          |                                                                            gATTAATCCTGAAAAAGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTc                                                                      >  2:230632/1‑81 (MQ=255)           gAAAAAGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCa                                                            >  1:230635/1‑81 (MQ=255)            aaaaaGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAAccc                                                             <  1:230611/79‑1 (MQ=255)                  tGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTGCGGTGACATATTCTGAGCTAGCTCGAACAACCCACTACTGc                                                     >  1:230641/1‑81 (MQ=255)                   ggACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGcc                                                    >  1:230651/1‑81 (MQ=255)                     aCGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGc                                                  >  2:230638/1‑81 (MQ=255)                        ccgcTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTaa                                               >  1:230649/1‑81 (MQ=255)                             gAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACggg                                           >  2:230656/1‑80 (MQ=255)                              aaGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGc                                          <  1:230613/80‑1 (MQ=255)                              aaGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCa                                         <  2:230612/81‑1 (MQ=255)                               aGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCAc                                        >  2:230660/1‑81 (MQ=255)                                    gcagcaAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGt                                   >  1:230655/1‑81 (MQ=255)                                            aTTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGc                           <  2:230614/81‑1 (MQ=255)                                             tttGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCt                          >  1:230640/1‑81 (MQ=255)                                                gttgCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTgc                        <  2:230615/80‑1 (MQ=255)                                                    cTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAg                   >  1:230647/1‑81 (MQ=255)                                                         ccGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCtgtg              >  2:230652/1‑81 (MQ=255)                                                             tGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATc          <  1:230616/81‑1 (MQ=255)                                                                 atatTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTg      <  2:230617/81‑1 (MQ=255)                                                                    ttCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGt   >  1:230645/1‑81 (MQ=255)                                                                     tCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGt   >  2:230646/1‑80 (MQ=255)                                                                      cTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTg  <  2:230618/80‑1 (MQ=255)                                                                          |                                                                            GATTAATCCTGAAAAAGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTG  >  NC_000913/1313391‑1313539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑