Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,320,045	G→T	A447E (GCA→GAA)	trpD ←	anthranilate synthase subunit TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,320,045	0	G	T	100.0%	92.0 / NA	26	A447E (GCA→GAA)	trpD	anthranilate synthase subunit TrpD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/17);  total (9/17)

GTTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCGTGTAA  >  NC_000913/1319967‑1320109                                                                               |                                                                 gtTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCg                                                                >  1:231500/1‑81 (MQ=255)    aaaaTGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGt                                                              >  2:231498/1‑80 (MQ=255)    aaaaTGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGt                                                              <  1:231468/80‑1 (MQ=255)     aaaTGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTg                                                             <  2:231469/80‑1 (MQ=255)       aTGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGc                                                          <  2:231470/81‑1 (MQ=255)             cGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAg                                                    <  1:231471/81‑1 (MQ=255)              ggTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGc                                                   <  1:231473/81‑1 (MQ=255)              ggTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGc                                                   <  2:231472/81‑1 (MQ=255)               gTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCATAGTCTTCTTCGGTGAGCTGATAGCt                                                  >  1:231501/1‑81 (MQ=255)                      tcCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAAtt                                           >  1:231504/1‑81 (MQ=255)                       ccGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTaa                                             <  1:231476/78‑1 (MQ=255)                       ccGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAAt                                            <  2:231475/79‑1 (MQ=255)                       ccGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAAt                                            <  1:231474/79‑1 (MQ=255)                            gtTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCg                                     >  2:231510/1‑81 (MQ=255)                             ttCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCg                                     <  2:231477/80‑1 (MQ=255)                                 gCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATg                                <  1:231478/81‑1 (MQ=255)                                   cTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATg                                >  1:231508/1‑79 (MQ=255)                                    tGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAg                             >  1:231503/1‑81 (MQ=255)                                     gCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGt                            >  1:231499/1‑81 (MQ=255)                                            gCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCa                      <  2:231479/80‑1 (MQ=255)                                                   gtggtAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGAtt                <  1:231481/79‑1 (MQ=255)                                                   gtggtAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGt              <  1:231480/81‑1 (MQ=255)                                                       tAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGgc          >  1:231506/1‑81 (MQ=255)                                                           gtgtCAGGCCAAAGTCTTCTTCGGTGGGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCgt      <  2:231482/81‑1 (MQ=255)                                                              tCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCgtgt    <  2:231483/80‑1 (MQ=255)                                                                aGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCGTGTaa  <  1:231484/80‑1 (MQ=255)                                                                               |                                                                 GTTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCGTGTAA  >  NC_000913/1319967‑1320109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑