Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,360	C→T	T47T (ACC→ACT)	yciV →	RNA/ssDNA 5'‑>3' exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,360	0	C	T	100.0%	57.4 / NA	17	T47T (ACC→ACT)	yciV	RNA/ssDNA 5'‑>3' exonuclease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/7);  total (10/7)

CTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACCACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCCCGGCGT  >  NC_000913/1323286‑1323431                                                                           |                                                                        cTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCt                                                                   >  2:231977/1‑81 (MQ=255)   gACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTg                                                                  >  1:231984/1‑80 (MQ=255)       ccAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATc                                                             <  1:231958/81‑1 (MQ=255)           aaGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCgcgc                                                         >  2:231978/1‑81 (MQ=255)             gCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCa                                                       <  1:231959/81‑1 (MQ=255)               aTTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGc                                                     <  1:231960/81‑1 (MQ=255)                          gTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGaaa                                          >  2:231980/1‑81 (MQ=255)                              aGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAAttt                                       >  2:231986/1‑80 (MQ=255)                                       gcgcGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCgg                              >  2:231990/1‑80 (MQ=255)                                                 aCCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTg                   <  1:231961/81‑1 (MQ=255)                                                  cccTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGa                  <  1:231962/81‑1 (MQ=255)                                                       gCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATc               <  1:231963/79‑1 (MQ=255)                                                       gCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCtt             <  2:231964/81‑1 (MQ=255)                                                        cGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTa            >  2:231991/1‑81 (MQ=255)                                                         gATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTAt           >  2:231985/1‑81 (MQ=255)                                                          aTCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTAtt          >  1:231989/1‑81 (MQ=255)                                                                  ccATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCCCGGCGt  >  1:231988/1‑81 (MQ=255)                                                                           |                                                                        CTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACCACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCCCGGCGT  >  NC_000913/1323286‑1323431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑