Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,328,943	T→C	N57S (AAC→AGC)	yciK ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,328,943	0	T	C	100.0%	44.6 / NA	14	N57S (AAC→AGC)	yciK	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/7);  total (7/7)

CAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGTTTATGTGGCTGGCTACCTGACGTAATTTTTCTTCATTACGGCCCAACAGAATCACTGTCGCACCATAGCGTG  >  NC_000913/1328867‑1329015                                                                             |                                                                         cAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGCTTAt                                                                      <  2:232690/81‑1 (MQ=255) cAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGCTTAt                                                                      <  2:232691/81‑1 (MQ=255)  aGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGCTTAtg                                                                     >  2:232719/1‑81 (MQ=255)       ttgGCAATTTTCGGACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGCTTATGtggct                                                                <  1:232692/81‑1 (MQ=255)           cAATTTTCGGACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGCTTATGTggctggct                                                            <  2:232693/81‑1 (MQ=255)               tttCGGACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGCTTATGTGGCTGGCTACCt                                                        >  1:232723/1‑81 (MQ=255)                   ggACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGCTTATGTGGCTGGCTACCTGACg                                                    >  1:232714/1‑81 (MQ=255)                   ggACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGCTTATGTGGCTGGCTACCTGACg                                                    <  2:232694/81‑1 (MQ=255)                             gTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGCTTATGTGGCTGGCTACCTGACGTAATTTttct                                          <  2:232695/81‑1 (MQ=255)                                                aCCACTGTGGCTGACGCCCAGTTTCTTCGCTTATGTGGCTGGCTACCTGACGTAATTTTTCTTCATTACGGCCCAACAGaa                       >  1:232729/1‑81 (MQ=255)                                                       tgGCTGACGCCCAGTTTCTTCGCTTATGTGGCTGGCTACCTGACGTAATTTTTCTTCATTACGGCCCAACAGAATCACTGt                <  2:232696/81‑1 (MQ=255)                                                          cTGACGCCCAGTTTCTTCGCTTATGTGGCTGGCTACCTGACGTAATTTTTCTTCATTACGGCCCAACAGAATCACTGTCg              >  2:232720/1‑80 (MQ=255)                                                            gACGCCCAGTTTCTTCGCTTATGTGGCTGGCTACCTGACGTAATTTTTCTTCATTACGGCCCAACAGAATCACTGTCGCAc           >  1:232722/1‑81 (MQ=255)                                                                      ttcttcGCTTATGTGGCTGGCTACCTGACGTAATTTTTCTTCATTACGGCCCAACAGAATCACTGTCGCACCATAGCGTg  >  1:232731/1‑80 (MQ=255)                                                                             |                                                                         CAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCGAGAATAAACCACTGTGGCTGACGCCCAGTTTCTTCGTTTATGTGGCTGGCTACCTGACGTAATTTTTCTTCATTACGGCCCAACAGAATCACTGTCGCACCATAGCGTG  >  NC_000913/1328867‑1329015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑