Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,340,717	G→A	A54T (GCG→ACG)	lapB →	lipopolysaccharide assembly protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,340,717	0	G	A	100.0%	60.3 / NA	18	A54T (GCG→ACG)	lapB	lipopolysaccharide assembly protein B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

CAACCGCTTGTCGCGTGATTACGTAGCGGGGGTTAACTTCCTGCTTAGTAATCAACAGGATAAAGCGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGAAACC  >  NC_000913/1340653‑1340789                                                                 |                                                                         cAACCGCTTGTCGCGTGATTACGTAGCGGGGGTTAACTTCCTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTtct                                                          <  2:234273/81‑1 (MQ=255)    ccGCTTGTCGCGTGATTACGTAGCGGGGGTTAACTTCCTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGa                                                       >  2:234291/1‑81 (MQ=255)          gTCGCGTGATTACGTAGCGGGGGTTAACTTCCTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGc                                                  <  1:234274/80‑1 (MQ=255)          gTCGCGTGATTACGTAGCGGGGGTTAACTTCCTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCt                                                 >  1:234294/1‑81 (MQ=255)          gTCGCGTGATTACGTAGCGGGGGTTAACTTCCTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCt                                                 >  1:234296/1‑81 (MQ=255)                 gATTACGTAGCGGGGGTTAACTTCCTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAagag                                          >  1:234300/1‑81 (MQ=255)                            gggggTTAACTTCCTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCAc                               <  2:234275/81‑1 (MQ=255)                                gTTAACTTCCTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGtt                           <  1:234276/81‑1 (MQ=255)                                        ccTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGcc                     <  2:234277/79‑1 (MQ=255)                                        ccTGCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGcc                     <  2:234278/79‑1 (MQ=255)                                           gCTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCt                >  1:234299/1‑81 (MQ=255)                                            cTTAGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCtt               <  1:234279/81‑1 (MQ=255)                                               aGTAATCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACg            >  1:234303/1‑81 (MQ=255)                                                  aaTCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTc         >  1:234305/1‑81 (MQ=255)                                                   aTCAACAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTc         <  2:234280/80‑1 (MQ=255)                                                      aaCAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGCGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGa      >  2:234304/1‑80 (MQ=255)                                                      aaCAGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGa      >  2:234308/1‑80 (MQ=255)                                                         aGGATAAAACGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGAAAcc  <  2:234281/81‑1 (MQ=255)                                                                 |                                                                         CAACCGCTTGTCGCGTGATTACGTAGCGGGGGTTAACTTCCTGCTTAGTAATCAACAGGATAAAGCGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGAAACC  >  NC_000913/1340653‑1340789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑