Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,371,451	G→T	L412F (TTG→TTT)	ycjM →	glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,371,451	0	G	T	100.0%	31.8 / NA	10	L412F (TTG→TTT)	ycjM	glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (3/7);  total (3/7)

ATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGT  >  NC_000913/1371375‑1371522                                                                             |                                                                        atatGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATtttttt                                                                       >  2:238435/1‑79 (MQ=255)    tGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTTTTAAGt                                                                   <  1:238412/80‑1 (MQ=255)        tGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTTTTAAGTTTccc                                                              <  2:238413/81‑1 (MQ=255)               aGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTTTTAAGTTTCCCCGGtgtg                                                       >  2:238433/1‑81 (MQ=255)               aGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTTTTAAGTTTCCCCGGtgtg                                                       >  2:238434/1‑81 (MQ=255)                 ccgccgTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTTTTAAGTTTCCCCGGTGTGcc                                                     <  1:238414/81‑1 (MQ=255)                               gCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTTTTAAGTTTCCCCGGTGTGCCAGCGATATATATTc                                       <  2:238415/81‑1 (MQ=255)                                         aCGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTTTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCt                             >  1:238430/1‑81 (MQ=255)                                                   aGGTTTATCCTTGACCATGCGATTTTTTTAAGTTTCCCCGGTGTGCCAGCGATATATATTAAAAGTATTCTTGGCTCGCGt                   <  1:238416/81‑1 (MQ=255)                                                         aTCCTTGCCCATGCGATTTTTTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGa              <  2:238417/80‑1 (MQ=255)                                                                    tGCGATTTTTTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGgtgt  <  1:238418/81‑1 (MQ=255)                                                                             |                                                                        ATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGT  >  NC_000913/1371375‑1371522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑