Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,375,016	A→G	E18E (GAA→GAG)	ycjQ →	putative zinc‑binding dehydrogenase YcjQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,375,016	0	A	G	100.0%	75.1 / NA	22	E18E (GAA→GAG)	ycjQ	putative zinc‑binding dehydrogenase YcjQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/7);  total (15/7)

CAGTTTCTTTACAAGGAGTTTTAAATGAAAAAGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAAGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTC  >  NC_000913/1374939‑1375091                                                                              |                                                                            cAGTTTCTTTACAAGGAGTTTTAAATGAAAAAGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGAt                                                                          >  2:238853/1‑81 (MQ=255)   gTTTCTTTACAAGGAGTTTTAAATGAAAAAGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCg                                                                        >  2:238857/1‑81 (MQ=255)    tttCTTTACAAGGAGTTTTAAATGAAAAAGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCgg                                                                       >  1:238847/1‑81 (MQ=255)     ttCTTTACAAGGAGTTTTAAATGAAAAAGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCggg                                                                      >  2:238849/1‑81 (MQ=255)                       aaaTGAAAAAGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAAtgaag                                                    >  1:238846/1‑81 (MQ=255)                          tGAAAAAGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAAtgaagtga                                                 <  2:238824/81‑1 (MQ=255)                               aaGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATc                                            >  1:238862/1‑81 (MQ=255)                                aGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATcc                                           >  1:238852/1‑81 (MQ=255)                                 gTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCcg                                          >  1:238854/1‑81 (MQ=255)                                  ttAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCcgc                                         >  2:238860/1‑81 (MQ=255)                                    agtagCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCgcgt                                       >  1:238863/1‑81 (MQ=255)                                     gtagCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCgcgt                                       <  1:238825/80‑1 (MQ=255)                                        gCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCgcgtgcgt                                   >  2:238856/1‑81 (MQ=255)                                        gCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCgcgtgcg                                    <  1:238826/80‑1 (MQ=255)                                         ccACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGtt                                  <  2:238827/81‑1 (MQ=255)                                               cACCGCGTGTTGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGgcg                            <  1:238828/81‑1 (MQ=255)                                                        ttGCTGCGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGaaa                    >  1:238855/1‑80 (MQ=255)                                                          gctgcGCTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAacac                 >  2:238867/1‑81 (MQ=255)                                                             gcgcTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACacgg               >  2:238859/1‑80 (MQ=255)                                                              cgcTGGTTGAGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACacgg               <  2:238829/79‑1 (MQ=255)                                                                       aGTATGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTgg    <  2:238830/81‑1 (MQ=255)                                                                          aTGAGGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTc  >  2:238881/1‑80 (MQ=255)                                                                              |                                                                            CAGTTTCTTTACAAGGAGTTTTAAATGAAAAAGTTAGTAGCCACAGCACCGCGTGTTGCTGCGCTGGTTGAGTATGAAGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTC  >  NC_000913/1374939‑1375091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑