Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,433	A→G	F32L (TTC→CTC)	fnr ←	DNA‑binding transcriptional dual regulator FNR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,433	0	A	G	100.0%	69.8 / NA	21	F32L (TTC→CTC)	fnr	DNA‑binding transcriptional dual regulator FNR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

CGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAA  >  NC_000913/1399356‑1399507                                                                              |                                                                           cGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCgg                                                                         >  2:242155/1‑81 (MQ=255)  gTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCggg                                                                        <  2:242122/81‑1 (MQ=255)    cTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGAt                                                                      >  2:242139/1‑81 (MQ=255)             cTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAgct                                                              <  1:242123/80‑1 (MQ=255)                aaTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAgctggct                                                          <  2:242125/81‑1 (MQ=255)                aaTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAgctgg                                                            <  2:242124/79‑1 (MQ=255)                 aTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAgctggct                                                          >  1:242156/1‑80 (MQ=255)                    ggCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGAtg                                                      <  2:242126/81‑1 (MQ=255)                    ggCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGAt                                                       <  1:242127/80‑1 (MQ=255)                           tcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAAt                                               >  2:242153/1‑81 (MQ=255)                             cGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATc                                              <  2:704329/80‑1 (MQ=255)                                 cAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTgg                                          >  2:242159/1‑80 (MQ=255)                                         ttatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGAt                                  <  2:242128/80‑1 (MQ=255)                                             cAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGca                              >  2:242154/1‑80 (MQ=255)                                             cAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGca                              >  2:242163/1‑80 (MQ=255)                                              aaGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGcac                             <  2:242129/80‑1 (MQ=255)                                                       aaGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGa                   <  1:242130/81‑1 (MQ=255)                                                           tCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTg                >  1:242161/1‑80 (MQ=255)                                                                    ttGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGt      <  2:242131/81‑1 (MQ=255)                                                                      gAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGta     <  1:242132/80‑1 (MQ=255)                                                                        gtgtGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATaa  >  1:242164/1‑81 (MQ=255)                                                                              |                                                                           CGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAA  >  NC_000913/1399356‑1399507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑