Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,809	A→G	intergenic (‑58/‑170)	mcaS ← / → smrA	small regulatory RNA McaS/DNA endonuclease SmrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,809	0	A	G	100.0%	38.4 / NA	13	intergenic (‑58/‑170)	mcaS/smrA	small regulatory RNA McaS/DNA endonuclease SmrA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/7);  total (6/7)

CCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAA  >  NC_000913/1405739‑1405874                                                                       |                                                                  cctcTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAatat                                                          >  1:242961/1‑80 (MQ=255)  ctctGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAata                                                           <  1:242948/78‑1 (MQ=255)        gcCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAATATAAGTTACa                                                  >  1:242958/1‑81 (MQ=255)               aCTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAATATAAGTTACATTATTaa                                           <  1:242949/81‑1 (MQ=255)                cTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAATATAAGTTACATTATTAat                                          <  2:242950/81‑1 (MQ=255)                  gtgtTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAATATAAGTTACATTATTAatat                                        >  1:242962/1‑81 (MQ=255)                       ataACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAATATAAGTTACATTATTAATATCGtg                                    <  2:242951/80‑1 (MQ=255)                           cGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAATATAAGTTACATTATTAATATCGtgaatga                               <  2:242952/81‑1 (MQ=255)                                ttAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAATATAAGTTACATTATTAATATCGTGAATGaataa                           <  2:242953/80‑1 (MQ=255)                                          gTTGCCAATTTTCTTCAGTGACAGATTTCGCGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAg                <  2:242954/81‑1 (MQ=255)                                             gCCAATTTTCTTCAGTGACAGATTTCGCGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTAt             >  1:242967/1‑81 (MQ=255)                                                     tcttcAGTGACAGATTTCGCGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCtt      >  2:242964/1‑80 (MQ=255)                                                         cAGTGACAGATTTCGCGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTataa  >  2:242965/1‑80 (MQ=255)                                                                       |                                                                  CCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAA  >  NC_000913/1405739‑1405874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑