Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,416,023	T→C	D455G (GAC→GGC)	recE ←	Rac prophage; exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,416,023	0	T	C	100.0%	56.2 / NA	18	D455G (GAC→GGC)	recE	Rac prophage; exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/9);  total (9/9)

TTACCCATTTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGTCATCGACAGGATTTTTTGATGGAATGTTTTTCCGGGCTTCATGGAGTTCTGCCCGCAGTTCCTGATAT  >  NC_000913/1415949‑1416091                                                                           |                                                                     ttACCCATTTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGa                                                                >  2:244164/1‑81 (MQ=255)     ccATTTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAg                                                             >  2:244171/1‑80 (MQ=255)      cATTTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGa                                                           <  2:244142/81‑1 (MQ=255)         ttCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGAttt                                                        >  1:244176/1‑81 (MQ=255)            ggATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGAtttttt                                                     >  1:244170/1‑81 (MQ=255)              aTCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGa                                                   <  1:244143/81‑1 (MQ=255)              aTCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGa                                                   >  2:244173/1‑81 (MQ=255)                     gggTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGATGGAATg                                            >  1:244160/1‑81 (MQ=255)                       gTCGCTAATTCCATCAACGAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGATGGAATGtt                                          <  1:244144/81‑1 (MQ=255)                               ttCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGATGGAATGTTTTTCCggg                                  >  1:244166/1‑81 (MQ=255)                               ttCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGATGGAATGTTTTTCCggg                                  <  2:244145/81‑1 (MQ=255)                                  cGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGATGGAATGTTTTTCCGGGCt                                <  2:244146/80‑1 (MQ=255)                                   gTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGATGGAATGTTTTTCCGGGCTTc                              <  1:244147/81‑1 (MQ=255)                                               accacGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGATGGAATGTTTTTCCGGGCTTCATGGAGTTCTGc                  >  2:244178/1‑81 (MQ=255)                                                   cGTGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGATGGAATGTTTTTCCGGGCTTCATGGAGTTCTGCCCGc              <  1:244148/81‑1 (MQ=255)                                                     tGATGCAGCAAGCAATTTATCGCCATCGACAGGATTTTTTGATGGAATGTTTTTCCGGGCTTCATGGAGTTCTGCCCGCAg            >  2:244204/1‑81 (MQ=255)                                                             caagcaaTTTATCGCCATCGACAGGATTTTTTGATGGAATGTTTTTCCGGGCTTCATGGAGTTCTGCCCGCAGTTCCTGat    <  2:244149/81‑1 (MQ=255)                                                               agcaaTTTATCGCCATCGACAGGATTTTTTGATGGAATGTTTTTCCGGGCTTCATGGAGTTCTGCCCGCAGTTCCTGatat  <  1:244150/81‑1 (MQ=255)                                                                           |                                                                     TTACCCATTTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGTCATCGACAGGATTTTTTGATGGAATGTTTTTCCGGGCTTCATGGAGTTCTGCCCGCAGTTCCTGATAT  >  NC_000913/1415949‑1416091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑