Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	163,469	C→T	G455G (GGC→GGT)	hrpB →	putative ATP‑dependent RNA helicase HrpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	163,469	0	C	T	100.0%	92.6 / NA	26	G455G (GGC→GGT)	hrpB	putative ATP‑dependent RNA helicase HrpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/14);  total (12/14)

GACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGCGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACGCTTAAACGTACGT  >  NC_000913/163395‑163541                                                                           |                                                                         gacgaAGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGggtgtggcg                                                                    >  2:35469/1‑81 (MQ=255)     aaGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGtttt                                                                >  2:35485/1‑81 (MQ=255)      aGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTc                                                               <  1:35431/81‑1 (MQ=255)                 cgGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAAc                                                    >  1:35479/1‑81 (MQ=255)                  ggCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAAcc                                                   <  1:35432/81‑1 (MQ=255)                  ggCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAAcc                                                   <  1:35434/81‑1 (MQ=255)                  ggCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAAcc                                                   <  1:35433/81‑1 (MQ=255)                  ggCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAAc                                                    >  1:35502/1‑80 (MQ=255)                     aaaaaTTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGc                                                >  1:35491/1‑81 (MQ=255)                             ccgccATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGgcag                                         >  1:35494/1‑80 (MQ=255)                              cgccATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGgcagca                                       <  2:35435/81‑1 (MQ=255)                                ccATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAAc                                     <  1:35436/81‑1 (MQ=255)                                        gAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTc                               <  1:35438/79‑1 (MQ=255)                                        gAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTc                               <  1:35439/79‑1 (MQ=255)                                        gAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTc                               <  2:35437/79‑1 (MQ=255)                                        gAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAg                             >  1:35493/1‑81 (MQ=255)                                        gAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAg                             >  1:35507/1‑81 (MQ=255)                                         aaGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAg                             >  1:35504/1‑80 (MQ=255)                                         aaGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAg                             >  1:35470/1‑80 (MQ=255)                                         aaGAGCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGc                            <  1:35440/81‑1 (MQ=255)                                          agagCCGCCACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGc                            >  2:35495/1‑80 (MQ=255)                                              ccaccACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTg                       <  1:35441/78‑1 (MQ=255)                                               cgccACGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGt                      >  2:35492/1‑81 (MQ=255)                                                   aCGGATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTaaa                  <  2:35442/81‑1 (MQ=255)                                                      gATGGGCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACg               >  2:35500/1‑81 (MQ=255)                                                          ggCAATAGTGACCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACGCtt            <  2:35443/80‑1 (MQ=255)                                                                    aCCTGGGTGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACGCTTAAacgtacgt  <  1:35444/80‑1 (MQ=255)                                                                           |                                                                         GACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGCGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACGCTTAAACGTACGT  >  NC_000913/163395‑163541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑