Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,425,909	A→C	I97L (ATC→CTC)	ydaY →	Rac prophage; putative uncharacterized protein YdaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,425,909	0	A	C	100.0%	86.8 / NA	26	I97L (ATC→CTC)	ydaY	Rac prophage; putative uncharacterized protein YdaY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/11);  total (15/11)

GTGTTGAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCC  >  NC_000913/1425832‑1425986                                                                              |                                                                              gtgtTGAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTc                                                                             <  1:245238/80‑1 (MQ=255)      gAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTg                                                                         >  1:245257/1‑79 (MQ=255)      gAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTg                                                                         >  2:245265/1‑79 (MQ=255)                tGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAagcagc                                                               >  2:245266/1‑79 (MQ=255)                 ggAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGcagccag                                                            >  1:245259/1‑81 (MQ=255)                  gAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGcagccag                                                            <  2:245239/80‑1 (MQ=255)                   aaTATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCa                                                          >  1:245262/1‑81 (MQ=255)                   aaTATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCa                                                          >  2:245263/1‑81 (MQ=255)                    atatACGGCAGAACGAGGGATCGCGCTGCTTAAGCGGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAACAt                                                         <  2:245240/81‑1 (MQ=255)                       taCGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGc                                                      >  2:245277/1‑81 (MQ=255)                         cGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGccc                                                    <  1:245241/81‑1 (MQ=255)                         cGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGccc                                                    <  2:245242/81‑1 (MQ=255)                              gAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAggg                                                >  2:245279/1‑80 (MQ=255)                               aaCGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGt                                               >  1:245272/1‑80 (MQ=255)                                  gAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTa                                              >  1:245267/1‑78 (MQ=255)                                      gATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGagca                                       <  2:245243/81‑1 (MQ=255)                                       aTCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGagcag                                      >  1:245276/1‑81 (MQ=255)                                       aTCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGagcag                                      <  1:245244/81‑1 (MQ=255)                                            gctgctTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGcagcag                                   >  1:245271/1‑79 (MQ=255)                                                ctTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAg                             >  1:245274/1‑81 (MQ=255)                                                 ttAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAg                             >  1:245273/1‑80 (MQ=255)                                                   aaGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGa                           >  2:245275/1‑80 (MQ=255)                                                             cAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATaaaaa                <  2:245245/81‑1 (MQ=255)                                                                agTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGc             <  1:245246/81‑1 (MQ=255)                                                                    gTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCAt          <  1:245247/80‑1 (MQ=255)                                                                           cAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGccc  <  2:245248/81‑1 (MQ=255)                                                                              |                                                                              GTGTTGAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCC  >  NC_000913/1425832‑1425986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑