Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,452,438	G→T	T402K (ACG→AAG)	paaZ ←	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,452,438	0	G	T	100.0%	83.8 / NA	24	T402K (ACG→AAG)	paaZ	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/12);  total (12/12)

CAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTG  >  NC_000913/1452362‑1452513                                                                             |                                                                            cAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGc                                                                         >  2:248503/1‑81 (MQ=255)    cGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGAc                                                                      <  1:248473/81‑1 (MQ=255)             aaGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCaaa                                                             <  2:248474/81‑1 (MQ=255)              aGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAg                                                            >  1:248498/1‑81 (MQ=255)                    ccgccgCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCtt                                                        <  2:248475/79‑1 (MQ=255)                      gccgccTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGt                                                    <  1:248476/81‑1 (MQ=255)                         gccTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTg                                                  >  1:248504/1‑80 (MQ=255)                         gccTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTg                                                  >  2:248502/1‑80 (MQ=255)                                 aaGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTAccg                                         >  2:248528/1‑81 (MQ=255)                                   gCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTAccgcc                                       >  1:248507/1‑81 (MQ=255)                                            agagCATGTCGCTGGTTTTGTGCTGGCATCGGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCa                              <  2:248477/81‑1 (MQ=255)                                            agagCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCa                              <  2:248478/81‑1 (MQ=255)                                              agCATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATc                            >  2:248514/1‑81 (MQ=255)                                                cATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCg                          <  2:248480/81‑1 (MQ=255)                                                cATGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCg                          <  1:248479/81‑1 (MQ=255)                                                 aTGTCGCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCgg                         >  2:248509/1‑81 (MQ=255)                                                      gCTGGTTTTGTGCTGGCATCAGCTTTGCGATAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCg                    >  2:248511/1‑81 (MQ=255)                                                      gCTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGc                     <  1:248481/80‑1 (MQ=255)                                                       cTGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCgg                   <  2:248483/81‑1 (MQ=255)                                                       atGGTTTTGTGCTGGCATCTGCTTTGCGACAGGGCAAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCgg                   <  2:248482/80‑1 (MQ=255)                                                        tGGTTTTGTGCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGa                  >  2:248506/1‑81 (MQ=255)                                                               gtgCTGGCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTAc            <  2:248484/80‑1 (MQ=255)                                                                     gCATCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAgg     >  2:248510/1‑81 (MQ=255)                                                                        tCAGCTTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTg  >  1:248519/1‑81 (MQ=255)                                                                             |                                                                            CAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTG  >  NC_000913/1452362‑1452513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑