Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,471,263	G→A	pseudogene (745/3495 nt)	ydbA →	putative outer membrane protein, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,471,263	0	G	A	100.0%	35.7 / NA	11	pseudogene (745/3495 nt)	ydbA	putative outer membrane protein, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

GTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATCAATATCAATGTTGGTACTGGTCAGGCGTTTTATAACGATGGCACAGGAACAATTGTTAACTATGGTACAATCTGCACTTTCGGCGTGTGCCAATC  >  NC_000913/1471188‑1471339                                                                            |                                                                             gTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATCAATATCAATGTTGGTACTAGTc                                                                           <  1:251011/79‑1 (MQ=255)   aGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATCAATATCAATGTTGGTACTAGTCAGGc                                                                       >  1:251034/1‑81 (MQ=255)    gggATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATCAATATCAATGTTGGTACTAGTCAGGCg                                                                      <  2:251012/81‑1 (MQ=255)            tGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATCAATATCAATGTTGGTACTAGTCAGGCGTTTTATaa                                                              >  1:251042/1‑81 (MQ=255)              tttATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATCAATATCAATGTTGGTACTAGTCAGGCGTTTTATAACg                                                            <  1:251013/81‑1 (MQ=255)                             cTGCTATCAACGACCAGACGGGTGTTATCAATATCAATGTTGGTACTAGTCAGGCGTTTTATAACGATGGCACAGGAACaa                                             <  2:251014/81‑1 (MQ=255)                                                      tatcaatatcaaTGTTGGTACTAGTCAGGCGTTTTATAACGATGGCACAGGAACAATTGTTAACTATGGTACAATCTGCAc                    >  2:251049/1‑81 (MQ=255)                                                          aatatcaatGTTGGTACTAGTCAGGCGTTTTATAACGATGGCACAGGAACAATTGTTAACTATGGTACAATCTGCACTTTc                <  1:251015/81‑1 (MQ=255)                                                                    ttGGTACTAGTCAGGCGTTTTATAACGATGGCACAGGAACAATTGTTAACTATGGTACAATCTGCACTTTCGGCGTGTGcc      <  1:251016/81‑1 (MQ=255)                                                                     tGGTACTAGTCAGGCGTTTTATAACGATGGCACAGGAACAATTGTTAACTATGGTACAATCTGCACTTTCGGCGTGTGcc      >  2:251043/1‑80 (MQ=255)                                                                        tACTAGTCAGGCGTTTTATAACGATGGCACAGGAACAATTGTTAACTATGGTACAATCTGCACTTTCGGCGTGTGCCAATc  >  1:251036/1‑81 (MQ=255)                                                                            |                                                                             GTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATCAATATCAATGTTGGTACTGGTCAGGCGTTTTATAACGATGGCACAGGAACAATTGTTAACTATGGTACAATCTGCACTTTCGGCGTGTGCCAATC  >  NC_000913/1471188‑1471339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑