Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,476,830	T→C	Y563H (TAT→CAT)	ydbD →	DUF2773 domain‑containing protein YdbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,476,830	0	T	C	100.0%	66.6 / NA	20	Y563H (TAT→CAT)	ydbD	DUF2773 domain‑containing protein YdbD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/11);  total (9/11)

GTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGAT  >  NC_000913/1476757‑1476894                                                                          |                                                                 gTCGATGAAACAAAAAATTTGGCTACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAAc                                                           <  1:251700/81‑1 (MQ=255)   cGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAAc                                                           >  1:251728/1‑79 (MQ=255)    gATGAAACAAAAAATTTGGCATCGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCa                                                         <  2:251704/80‑1 (MQ=255)    gATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAAcaa                                                         <  2:251701/80‑3 (MQ=255)    gATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCa                                                         <  1:251702/80‑1 (MQ=255)    gATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCa                                                         <  1:251703/80‑1 (MQ=255)    gATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAg                                                        >  1:251726/1‑81 (MQ=255)     aTGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAgc                                                       >  1:251721/1‑81 (MQ=255)     aTGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAgc                                                       >  1:251724/1‑81 (MQ=255)             aaaaaTTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGatat                                               <  1:251705/81‑1 (MQ=255)                       cAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCt                                     >  1:251732/1‑81 (MQ=255)                           gTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGc                                 <  2:251706/81‑1 (MQ=255)                                     gCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCa                       >  2:251722/1‑81 (MQ=255)                                       aTCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGTTGATATGCTTTTGTCTGAGCTGATTAGCCACg                     >  1:251730/1‑81 (MQ=255)                                          ggAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGc                   <  1:251707/80‑1 (MQ=255)                                          ggAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGc                   >  1:251737/1‑80 (MQ=255)                                            aaaaaTAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGaa                <  1:251708/81‑1 (MQ=255)                                                   atCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTtgatg         <  2:251709/81‑1 (MQ=255)                                                   atCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTtgat          >  1:251729/1‑80 (MQ=255)                                                          aGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGAt  <  1:251710/81‑1 (MQ=255)                                                                          |                                                                 GTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGAT  >  NC_000913/1476757‑1476894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑