| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,493,606 | T→C | I379I (ATT→ATC) | trg → | methyl‑accepting chemotaxis protein Trg |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,493,606 | 0 | T | C | 96.3% | 90.1 / ‑3.0 | 27 | I379I (ATT→ATC) | trg | methyl‑accepting chemotaxis protein Trg |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (14/12); minor base G (1/0); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
GGTGTAGTAAAAACGATGGGCGCTATCTCCACGAGTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATTGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGTGAGCAAGGGCGTGGATTT > NC_000913/1493529‑1493681 | ggTGTAGTAAAAACGATGGGCGCTATCTCCACGAGTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCt > 1:253710/1‑81 (MQ=255) gtgtAGTAAAAACGATGGGCGCTATCTCCACGAGTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCtt > 2:253713/1‑81 (MQ=255) taAAAACGATGGGCGCTATCTCCACGAGTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGa > 1:253698/1‑81 (MQ=255) gggCGCTATCTCCACGAGTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCt > 1:253720/1‑81 (MQ=255) tctcCACGAGTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTg < 1:253675/80‑1 (MQ=255) tctcCACGAGTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAAAATTCTGGCACTg < 2:253676/80‑1 (MQ=255) ctcCACGAGTTCGAAGAAACTTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGaa > 1:253717/1‑81 (MQ=255) ccACGAGTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAAtg > 1:253714/1‑81 (MQ=255) gTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCg > 1:253722/1‑81 (MQ=255) ttCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGt < 1:253677/81‑1 (MQ=255) cGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGtt < 2:253678/80‑1 (MQ=255) gaagaaAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTg > 1:253721/1‑80 (MQ=255) aagaaAATTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTg > 1:253719/1‑79 (MQ=255) aTTTCTGAGATCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCcgcg > 1:253715/1‑81 (MQ=255) agaTCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGc < 2:253682/79‑1 (MQ=255) agaTCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCgg < 1:253679/81‑1 (MQ=255) agaTCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCg < 1:253681/80‑1 (MQ=255) agaTCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAACGCTGCCGTTGAAGCCGCGCGAGCg < 2:253680/80‑1 (MQ=255) gaTCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCggg < 1:253683/81‑1 (MQ=255) aTCACCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGt > 2:253716/1‑81 (MQ=255) aCCGCCGTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGTGa < 2:253684/80‑1 (MQ=255) cgccgTCATCAACAGTATGGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGTGAGc > 1:253718/1‑80 (MQ=255) ccgTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGTGAGCAAg > 2:253723/1‑81 (MQ=255) gTCATCAACAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGTGAGCAAggg > 2:253712/1‑81 (MQ=255) tcatcaACAGTATCGCTTGCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGTGAGCAAGGGc < 2:253685/81‑1 (MQ=255) aaCAGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGTGAGCAAGGGCGTgg < 1:253686/80‑1 (MQ=255) aGTATCGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGTGAGCAAGGGCGTGGAttt > 2:253726/1‑81 (MQ=255) | GGTGTAGTAAAAACGATGGGCGCTATCTCCACGAGTTCGAAGAAAATTTCTGAGATCACCGCCGTCATCAACAGTATTGCTTTCCAGACGAATATTCTGGCACTGAATGCTGCCGTTGAAGCCGCGCGAGCGGGTGAGCAAGGGCGTGGATTT > NC_000913/1493529‑1493681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |