Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,463	C→T	T203I (ACT→ATT)	opgD →	glucan biosynthesis protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,463	0	C	T	100.0%	39.9 / NA	12	T203I (ACT→ATT)	opgD	glucan biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/8);  total (4/8)

TCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCT  >  NC_000913/1497386‑1497540                                                                              |                                                                              tctcGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACAttt                                                                             <  2:254183/80‑1 (MQ=255)     ggCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACATTTac                                                                           <  1:254184/78‑1 (MQ=255)     ggCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACATTTACAcc                                                                        >  1:254204/1‑81 (MQ=255)        gcgAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACATTTACACCGAc                                                                     <  2:254185/81‑1 (MQ=255)             ttATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACATTTACACCGACAGTaa                                                                <  1:254186/81‑1 (MQ=255)                   ccGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACATTTACACCGACAGTAaagaag                                                           <  1:254187/80‑1 (MQ=255)                           ttGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACATTTACACCGACAGTAAAGAAGAGTTcccc                                                   >  1:254208/1‑80 (MQ=255)                                    cATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACATTTACACCGACAGTAAAGAAGAGTACCCCGACTTTACCg                                         <  1:254188/81‑1 (MQ=255)                                                       ggCCCGCGGCCTGGCGATCGACATTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACgg                       <  1:254189/80‑1 (MQ=255)                                                           cgcgGCCTGGCGATCGACATTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTaaaa                  <  2:254190/81‑1 (MQ=255)                                                                   ggCGATCTACATTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCggggg           >  1:254207/1‑80 (MQ=255)                                                                    gCGATCGACATTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCa         <  2:254191/81‑1 (MQ=255)                                                                       aTCGACATTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAAc       >  1:254211/1‑80 (MQ=255)                                                                           acatTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCt  >  1:254209/1‑81 (MQ=255)                                                                              |                                                                              TCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCT  >  NC_000913/1497386‑1497540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑