Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,531,277	G→A	pseudogene (692/1054 nt)	yncI →	putative transposase YncI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,531,277	0	G	A	100.0%	24.2 / NA	8	pseudogene (692/1054 nt)	yncI	putative transposase YncI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

CATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGAACTTTTGTGATTTTGAATTCGAATGG  >  NC_000913/1531199‑1531348                                                                               |                                                                        cATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGaaa                                                                       <  2:258261/81‑1 (MQ=255)     cGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGaaagaa                                                                    <  2:258262/80‑1 (MQ=255)                      tAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGAAAGAAAAGAAACACGTTTGCat                                                   <  1:258263/80‑1 (MQ=255)                          tgtgTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGAAAGAAAAGAAACACGTTTGCATATTGt                                              <  1:258264/81‑1 (MQ=255)                           gtgtTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGAAAGAAAAGAAACACGTTTGCATATTGt                                              >  2:342185/1‑80 (MQ=255)                                    aaTTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGAAAGAAAAGAAACACGTTTGCATATTGTCAgtaacgt                                     >  1:258276/1‑80 (MQ=255)                                             ggCGATTCGTTTAGTACGCAGGAGATAAGTCATGAAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGaa                           <  2:258265/81‑1 (MQ=255)                                                     gTTTAGTACGCAGGAGATAAGTCATGAAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGAACTTTtgt                    <  1:258266/80‑1 (MQ=255)                                                          gTACGCAGGAGATAAGTCATGAAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGAACTTTTGTGAtttt              <  1:258267/81‑1 (MQ=255)                                                                       aaGTCATGAAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGAACTTTTGTGATTTTGAATTCGAATgg  >  2:258277/1‑80 (MQ=255)                                                                               |                                                                        CATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGAACTTTTGTGATTTTGAATTCGAATGG  >  NC_000913/1531199‑1531348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑