Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,551,332	A→C	D291A (GAT→GCT)	fdnH →	formate dehydrogenase N subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,551,332	0	A	C	100.0%	56.2 / NA	17	D291A (GAT→GCT)	fdnH	formate dehydrogenase N subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/8);  total (9/8)

GCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCGTGGTGATT  >  NC_000913/1551268‑1551406                                                                 |                                                                           gCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGt                                                             <  1:260541/80‑1 (MQ=255)  ccGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCg                                                           <  1:260542/81‑1 (MQ=255)         gATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATg                                                     <  2:260543/80‑1 (MQ=255)         gATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGa                                                    >  1:260563/1‑81 (MQ=255)                cACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTgcg                                             >  1:260564/1‑81 (MQ=255)                cACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTgcg                                             >  1:260565/1‑81 (MQ=255)                         ggTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAAtt                                    >  1:260562/1‑81 (MQ=255)                           tATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTa                                  <  1:260544/81‑1 (MQ=255)                                gCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGAt                             >  2:260573/1‑81 (MQ=255)                                gCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGAt                             <  1:260545/81‑1 (MQ=255)                                 cccGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATc                            >  1:260572/1‑81 (MQ=255)                                 cccGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATc                            >  2:260566/1‑81 (MQ=255)                                      aTAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGcc                       <  2:260546/81‑1 (MQ=255)                                         aGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTg                     <  2:260547/80‑1 (MQ=255)                                             aGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACt                <  2:260548/81‑1 (MQ=255)                                                 gACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGAc            >  1:260570/1‑81 (MQ=255)                                                           aggaggCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCGTGGTGAtt  >  2:260568/1‑81 (MQ=255)                                                                 |                                                                           GCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCGTGGTGATT  >  NC_000913/1551268‑1551406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑