Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,561,398	A→G	Y362H (TAC→CAC)	ddpA ←	putative D,D‑dipeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,561,398	0	A	G	100.0%	78.8 / NA	23	Y362H (TAC→CAC)	ddpA	putative D,D‑dipeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/8);  total (15/8)

CAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTA  >  NC_000913/1561323‑1561473                                                                            |                                                                            caATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGaa                                                                        <  2:261757/81‑1 (MQ=255)  aaTGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGaaa                                                                       >  1:261779/1‑81 (MQ=255)   atgatgCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAAc                                                                      >  1:261781/1‑81 (MQ=255)      atgCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGt                                                                    >  2:261787/1‑80 (MQ=255)      atgCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGt                                                                    >  2:261782/1‑80 (MQ=255)       tgCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCa                                                                  <  2:261758/81‑1 (MQ=255)           cAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCt                                                              >  1:261783/1‑81 (MQ=255)            aGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCtg                                                             >  2:261775/1‑81 (MQ=255)            aGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCtg                                                             <  2:261759/81‑1 (MQ=255)                 gttgAGACTGGATTGTGTCGCAAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCtggtgg                                                         <  1:261760/80‑1 (MQ=255)                    gagaCTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGttt                                                     >  1:261777/1‑81 (MQ=255)                     agaCTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGttt                                                     <  1:261761/80‑1 (MQ=255)                           gATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCg                                                <  2:261762/79‑1 (MQ=255)                               gtgtCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACt                                           <  1:261763/80‑1 (MQ=255)                                 gtCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTa                                        >  1:261785/1‑81 (MQ=255)                                  tCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTAt                                       >  2:261780/1‑81 (MQ=255)                                      cAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCa                                   >  2:261778/1‑81 (MQ=255)                                               aGGCTCCCAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCggcttt                          >  2:261786/1‑81 (MQ=255)                                                     ccAGTTCGGATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCggctttggcttt                    >  2:261792/1‑81 (MQ=255)                                                            ggATCGTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCtttcgtttcg             >  1:261784/1‑81 (MQ=255)                                                                 gTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCAt         <  1:261764/80‑1 (MQ=255)                                                                 gTTATCGGAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATg        >  1:261806/1‑81 (MQ=255)                                                                       ggAGTGGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTa  >  2:261794/1‑81 (MQ=255)                                                                            |                                                                            CAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTA  >  NC_000913/1561323‑1561473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑