Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,575,336	T→G	S762R (AGC→CGC)	yddB ←	putative TonB‑dependent receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,575,336	0	T	G	100.0%	45.2 / NA	14	S762R (AGC→CGC)	yddB	putative TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/8);  total (6/8)

TGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCC  >  NC_000913/1575258‑1575401                                                                               |                                                                  tGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGAt                                                                 <  1:263341/81‑1 (MQ=255)  gCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATc                                                                >  2:263360/1‑81 (MQ=255)      aCATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGt                                                            <  1:263342/81‑1 (MQ=255)                        cGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCa                                          >  2:263356/1‑81 (MQ=255)                          gCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACt                                        <  2:263343/81‑1 (MQ=255)                           ccACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACt                                        >  1:263364/1‑80 (MQ=255)                             aCTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGAt                                     <  2:263344/81‑1 (MQ=255)                                       gTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGttt                           <  1:263345/81‑1 (MQ=255)                                                ccGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgttgttg                   >  1:263362/1‑80 (MQ=255)                                                ccGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGc                  <  1:263346/81‑1 (MQ=255)                                                         gTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATtgt         >  2:263361/1‑81 (MQ=255)                                                            gTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTgg       <  1:263347/80‑1 (MQ=255)                                                            gTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGc      >  1:263363/1‑81 (MQ=255)                                                                 aaCAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTcc  <  2:531033/80‑1 (MQ=255)                                                                               |                                                                  TGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCC  >  NC_000913/1575258‑1575401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑