Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,578,871	A→G	F158L (TTC→CTC)	yddA ←	ABC transporter family protein YddA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,578,871	0	A	G	100.0%	74.6 / NA	22	F158L (TTC→CTC)	yddA	ABC transporter family protein YddA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

GTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATC  >  NC_000913/1578798‑1578943                                                                          |                                                                         gTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAAt                                                                   <  1:263683/81‑1 (MQ=255)    aaTGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGa                                                                 >  1:263716/1‑80 (MQ=255)                             aaaGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGt                                        >  2:263717/1‑80 (MQ=255)                               aGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGt                                        >  2:263719/1‑78 (MQ=255)                                  aTAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAagag                                   >  2:263709/1‑80 (MQ=255)                                       ggTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTc                             <  1:263684/81‑1 (MQ=255)                                         tAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCtt                           <  1:263685/81‑1 (MQ=255)                                           aaGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAGAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCa                         <  2:263686/81‑1 (MQ=255)                                           aaGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCa                         <  1:263688/81‑1 (MQ=255)                                           aaGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCa                         <  2:263687/81‑1 (MQ=255)                                                 aTCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGAt                    <  2:350407/80‑1 (MQ=255)                                                 aTCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGAt                    <  1:263689/80‑1 (MQ=255)                                                    aGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGt                >  1:263714/1‑81 (MQ=255)                                                    aGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGt                <  1:263690/81‑1 (MQ=255)                                                     gCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGtt               >  1:263711/1‑81 (MQ=255)                                                       aTGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTgg             >  1:263706/1‑81 (MQ=255)                                                       aTGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTgg             <  1:263691/81‑1 (MQ=255)                                                        tGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGt            >  2:263715/1‑81 (MQ=255)                                                          cTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGt            >  1:263721/1‑79 (MQ=255)                                                          cTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc           <  2:263692/80‑1 (MQ=255)                                                             aGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAg         <  2:263693/79‑1 (MQ=255)                                                                  cTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATc  >  1:263727/1‑81 (MQ=255)                                                                          |                                                                         GTGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATC  >  NC_000913/1578798‑1578943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑