Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,585,441	G→C	A349G (GCC→GGC)	ydeP ←	putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,585,441	0	G	C	100.0%	69.2 / NA	21	A349G (GCC→GGC)	ydeP	putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/9);  total (12/9)

CTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGT  >  NC_000913/1585374‑1585512                                                                    |                                                                        cTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTg                                                            <  1:264335/81‑1 (MQ=255)             cATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAg                                                 >  2:264359/1‑80 (MQ=255)                  cGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATAc                                           <  2:264336/81‑1 (MQ=255)                       cAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCg                                      <  2:264337/81‑1 (MQ=255)                        aGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGa                                     >  1:264353/1‑81 (MQ=255)                            aaTGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATc                                 >  1:264357/1‑81 (MQ=255)                            aaTGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATc                                 >  1:264367/1‑81 (MQ=255)                              tGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCtt                               >  1:264356/1‑81 (MQ=255)                               ggTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCttt                              >  1:264368/1‑81 (MQ=255)                                    gTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACt                         <  1:264338/81‑1 (MQ=255)                                    gTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACt                         >  2:264355/1‑81 (MQ=255)                                         gCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGaa                    >  1:264364/1‑81 (MQ=255)                                         gCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGaa                    >  1:264361/1‑81 (MQ=255)                                           aGCGGCATATGCGTCAGCCAGTTCGCCGATTAGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAAtt                  <  2:264339/81‑1 (MQ=255)                                                 atatGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAAc            <  2:264340/81‑1 (MQ=255)                                                    tGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTc         <  2:264341/81‑1 (MQ=255)                                                         cAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGc    >  1:264373/1‑81 (MQ=255)                                                          aGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGc    <  1:264342/80‑1 (MQ=255)                                                           gCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCg   >  2:264363/1‑80 (MQ=255)                                                           gCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGt  <  2:264343/81‑1 (MQ=255)                                                            ccAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCg   >  1:264362/1‑79 (MQ=255)                                                                    |                                                                        CTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGT  >  NC_000913/1585374‑1585512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑