Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,603,093	T→C	G25G (GGT→GGC)	lsrC →	Autoinducer‑2 ABC transporter membrane subunit LsrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,603,093	0	T	C	100.0%	71.1 / NA	22	G25G (GGT→GGC)	lsrC	Autoinducer‑2 ABC transporter membrane subunit LsrC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/15);  total (7/15)

GTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGG  >  NC_000913/1603016‑1603169                                                                              |                                                                             gTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGcttt                                                                           >  2:266339/1‑81 (MQ=255)  tCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTtc                                                                          <  1:266310/81‑1 (MQ=255)    aTGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTtctc                                                                        >  2:266336/1‑81 (MQ=255)     tGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCg                                                                       <  2:266311/81‑1 (MQ=255)       cTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGAc                                                                     <  2:266312/81‑1 (MQ=255)          aaGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGACCGc                                                                  <  1:266313/81‑1 (MQ=255)                 ttCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGACCGCCAGTAtt                                                           <  2:266314/81‑1 (MQ=255)                    aGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGACCGCCAGTATTTa                                                         <  1:266315/80‑1 (MQ=255)                      aacaacCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAgt                                                      <  2:266316/81‑1 (MQ=255)                       acaacCGTGAAATCACGGCACTGCTGTCGGTGGTGCTGCTGTCTGTATTACCCTGCTTTCTCAACCGCCCGTATTTAAgtg                                                     >  1:266341/1‑81 (MQ=255)                             gTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCaaa                                               >  1:266345/1‑81 (MQ=255)                                 aaTCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCt                                           <  2:266317/81‑1 (MQ=255)                                         caatgctgGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATgg                                   <  2:266318/78‑1 (MQ=255)                                             gctgGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTta                               >  2:266348/1‑81 (MQ=255)                                                  cGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATagca                          <  2:266319/81‑1 (MQ=255)                                                             ctgTTTGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatc               >  2:266347/1‑81 (MQ=255)                                                             ctgTTTGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAat                <  1:266320/80‑1 (MQ=255)                                                                tttGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcct             <  2:266321/80‑1 (MQ=255)                                                                  tGTATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcctga           <  1:266322/80‑1 (MQ=255)                                                                    tATTACCCGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcctgatc         <  2:266323/80‑1 (MQ=255)                                                                      ttACCCGGCTTTCTCGACCGCTAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcctgatcct       <  1:266324/80‑1 (MQ=255)                                                                          ccGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTgg  >  2:266351/1‑81 (MQ=255)                                                                          ccGGCTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTgg  >  2:266353/1‑81 (MQ=255)                                                                              |                                                                             GTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGG  >  NC_000913/1603016‑1603169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑