Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,607,828	C→T	G161G (GGC→GGT)	tam →	trans‑aconitate 2‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,607,828	0	C	T	100.0%	66.9 / NA	20	G161G (GGC→GGT)	tam	trans‑aconitate 2‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

CCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCA  >  NC_000913/1607758‑1607902                                                                       |                                                                           cccATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGTCGTGAGCCGTTGGCTGGTGTTCATGCt                                                                   >  2:266890/1‑80 (MQ=255)         cTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGat                                                          <  1:266867/81‑1 (MQ=255)            aTGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGatat                                                        <  2:266868/80‑1 (MQ=255)             tGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATAttt                                                      <  1:266869/81‑1 (MQ=255)             tGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATAttt                                                      >  2:266886/1‑81 (MQ=255)               cgcgAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTTGTGTTCATGCTTACTACGATATTTTg                                                    <  1:266871/81‑1 (MQ=255)               cgcgAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATAtttt                                                     <  2:266870/80‑1 (MQ=255)                              gAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGAtgt                                     <  1:266872/81‑1 (MQ=255)                              gAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGAtgt                                     >  2:266893/1‑81 (MQ=255)                                   aaaCTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGt                                <  2:266873/81‑1 (MQ=255)                                        aCCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGata                           >  1:266889/1‑81 (MQ=255)                                        aCCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGata                           >  2:266898/1‑81 (MQ=255)                                        aCCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGata                           >  2:266902/1‑81 (MQ=255)                                           cGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCt                        <  1:266874/81‑1 (MQ=255)                                                   gggCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac                 >  1:266895/1‑80 (MQ=255)                                                   gggCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac                 >  2:266901/1‑80 (MQ=255)                                                   gggCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac                 >  2:266903/1‑80 (MQ=255)                                                            ccGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTAt       >  1:266896/1‑81 (MQ=255)                                                            ccGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTAt       >  2:266888/1‑81 (MQ=255)                                                               ttGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAc    <  2:330897/81‑1 (MQ=255)                                                                 ggctggtGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAcc   <  1:266875/80‑1 (MQ=255)                                                                 ggctggtGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAcc   <  2:266876/80‑1 (MQ=255)                                                                  gctggtGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCa  <  1:266877/80‑1 (MQ=255)                                                                       |                                                                           CCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCA  >  NC_000913/1607758‑1607902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑