Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,658,706 G→C G213A (GGC→GCC)  ynfE → putative selenate reductase YnfE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,658,7060GC91.7% 32.5 / ‑2.7 12G213A (GGC→GCC) ynfEputative selenate reductase YnfE
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (6/5);  total (6/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

TTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACC  >  NC_000913/1658629‑1658773
                                                                             |                                                                   
ttCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCaa                                                                  >  2:272357/1‑81 (MQ=255)
           aGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACgg                                                       <  2:272338/81‑1 (MQ=255)
             tATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGa                                                      >  2:272362/1‑80 (MQ=255)
                     cTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGaa                                               <  2:272339/79‑1 (MQ=255)
                                          cTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGt                         <  2:272340/80‑1 (MQ=255)
                                                     ccTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAAcc             >  2:272366/1‑81 (MQ=255)
                                                         cacCTACGGCAGTAATGATGGCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCgg          <  1:272343/80‑1 (MQ=255)
                                                         cacCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCg           <  2:272341/79‑1 (MQ=255)
                                                         cacCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGc         <  1:272342/81‑1 (MQ=255)
                                                          acCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCgg          >  2:272364/1‑79 (MQ=255)
                                                              aCGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGaaa    >  1:272365/1‑81 (MQ=255)
                                                                ggCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAAcc  >  1:272376/1‑81 (MQ=255)
                                                                             |                                                                   
TTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACC  >  NC_000913/1658629‑1658773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑