| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,682,286 | G→A | R43Q (CGA→CAA) | rstA → | DNA‑binding transcriptional regulator RstA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,682,286 | 0 | G | A | 100.0% | 53.8 / NA | 16 | R43Q (CGA→CAA) | rstA | DNA‑binding transcriptional regulator RstA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/8); total (8/8) | |||||||||||
CCGCGTACCTGGCAAAACATGATATGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGC > NC_000913/1682211‑1682362 | ccGCGTACCTGGCAAAACATGATATGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGaa > 2:275244/1‑80 (MQ=255) cgTACCTGGCAAAACATGATATGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATc < 1:275228/81‑1 (MQ=255) cgTACCTGGCAAAACATGATATGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATc < 2:275227/81‑1 (MQ=255) atatGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTc < 2:275229/80‑1 (MQ=255) tACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGc > 1:275262/1‑80 (MQ=255) aCCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCt < 2:275230/80‑1 (MQ=255) gagCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGc < 2:275231/81‑1 (MQ=255) gCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATCTGGTGTTACTCGACATCATGCTACCAGGc > 2:275248/1‑79 (MQ=255) cgACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCAt < 1:275232/81‑1 (MQ=255) ccAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGAc < 1:275233/81‑1 (MQ=255) cGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTg > 2:275256/1‑81 (MQ=255) gaaACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGt > 1:275254/1‑81 (MQ=255) gaaACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGt < 2:275234/81‑1 (MQ=255) aaaCCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGt > 1:275250/1‑80 (MQ=255) aaaCCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGt > 2:275257/1‑80 (MQ=255) ttGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTAcgc > 1:275259/1‑81 (MQ=255) | CCGCGTACCTGGCAAAACATGATATGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGC > NC_000913/1682211‑1682362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |