| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,686,411 | C→T | E60K (GAA→AAA) | fumC ← | fumarase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,686,411 | 0 | C | T | 93.3% | 46.5 / ‑6.0 | 15 | E60K (GAA→AAA) | fumC | fumarase C |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/6); minor base G (1/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAAT > NC_000913/1686334‑1686486 | aTGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTTAtt < 1:275738/81‑1 (MQ=255) gCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTTATTaa > 2:275768/1‑81 (MQ=255) tCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTTATTAACt < 1:275739/79‑1 (MQ=255) tGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTTATTAACTTTTGc > 2:275775/1‑81 (MQ=255) aGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTTATTAACTTTTGCCGCt < 1:275740/81‑1 (MQ=255) gccgccTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTTATTAACTTTTGCCGCTGCACGCTTGGtt < 1:275741/81‑1 (MQ=255) gccgccTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTTATTAACTTTTGCCGCTGCACGCTTGGt > 1:275753/1‑80 (MQ=255) cTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTGATTAACTTTTGCCGCTGCACGCTTGGTTAgcgc > 2:275765/1‑81 (MQ=255) aaTGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTTATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAgcgc < 1:275742/81‑1 (MQ=255) ctTCAGACAACAAGCCTAAATCTTTATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTgg > 2:275770/1‑81 (MQ=255) aGACAACAAGCCTAAATCTTTATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAt > 2:275762/1‑81 (MQ=255) aacaaGCCTAAATCTTTATTAACTTTTGCCGCTGCACGCTTGGTTAGCGTCAGCGCATGAATCAGTGAGGTGGGCATTTtc > 2:275763/1‑81 (MQ=255) caaGCCTAAATCTTTATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTtctc < 1:275743/81‑1 (MQ=255) aaGCCTAAATCTTTATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTcc > 1:275764/1‑81 (MQ=255) aTCTTTATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAAt > 1:275767/1‑81 (MQ=255) | ATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAAT > NC_000913/1686334‑1686486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |