Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	182,578	G→A	G39D (GGC→GAC)	cdaR →	DNA‑binding transcriptional activator CdaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	182,578	0	G	A	100.0%	141.0 / NA	40	G39D (GGC→GAC)	cdaR	DNA‑binding transcriptional activator CdaR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/19);  total (21/19)

TCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAGTCGTCGATATCGAT  >  NC_000913/182503‑182654                                                                            |                                                                             tCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATc                                                                         <  1:39523/81‑1 (MQ=255) tCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATc                                                                         >  2:39570/1‑81 (MQ=255) tCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATc                                                                         <  1:39522/81‑1 (MQ=255) tCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATc                                                                         <  2:39524/81‑1 (MQ=255)   gTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGt                                                                       >  2:39596/1‑81 (MQ=255)   gTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGt                                                                       >  2:39595/1‑81 (MQ=255)       cACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGc                                                                   >  2:39566/1‑81 (MQ=255)             ccATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTAtt                                                              <  2:39525/80‑1 (MQ=255)               aTGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGt                                                           >  2:39569/1‑81 (MQ=255)                        atcGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCAc                                                  <  1:39526/81‑1 (MQ=255)                           gATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGa                                                <  2:39527/80‑1 (MQ=255)                               ccAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTg                                           <  2:39529/81‑1 (MQ=255)                               ccAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTg                                           <  1:39528/81‑1 (MQ=255)                                 aaTATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCa                                         >  1:39579/1‑81 (MQ=255)                                     tCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGc                                     <  1:39530/81‑1 (MQ=255)                                       aaCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCt                                    <  1:39531/80‑1 (MQ=255)                                         cGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTgg                                  >  2:39572/1‑80 (MQ=255)                                          gTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTa                                >  2:39588/1‑81 (MQ=255)                                          gTAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTa                                >  2:39567/1‑81 (MQ=255)                                           tAATGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTAc                               >  1:39584/1‑81 (MQ=255)                                            aaTGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACt                              >  2:39575/1‑81 (MQ=255)                                             aTGGATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACtt                             <  2:39532/81‑1 (MQ=255)                                               ggATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACttt                            >  1:39594/1‑80 (MQ=255)                                                gATGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTca                          <  1:39533/81‑1 (MQ=255)                                                  tGCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTca                          >  1:39592/1‑79 (MQ=255)                                                   gCCCGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAg                       >  1:39590/1‑81 (MQ=255)                                                    cccGTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAg                       <  2:39534/80‑1 (MQ=255)                                                       gTGGGCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACATGGAc                   >  2:39606/1‑81 (MQ=255)                                                          ggCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAg                >  2:39600/1‑81 (MQ=255)                                                          ggCGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAg                >  2:39604/1‑81 (MQ=255)                                                            cGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAgtc              <  1:39535/81‑1 (MQ=255)                                                            cGAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAgtc              <  1:39536/81‑1 (MQ=255)                                                             gAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAgtcg             <  2:39537/81‑1 (MQ=255)                                                             gAATTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAgtcg             >  1:39598/1‑81 (MQ=255)                                                               aTTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAgtcgtc           <  2:39538/81‑1 (MQ=255)                                                               aTTATCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAgtcgtc           <  1:39539/81‑1 (MQ=255)                                                                  aTCGGCAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAGTCGTCGa         >  1:39601/1‑80 (MQ=255)                                                                       cAGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAGTCGTCGATATCGa   <  2:39540/81‑1 (MQ=255)                                                                        aGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAGTCGTCGATATCGAt  >  2:39573/1‑81 (MQ=255)                                                                        aGCGACGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAGTCGTCGATATCGAt  >  2:39599/1‑81 (MQ=255)                                                                            |                                                                             TCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACGAAGGTGCATTGCTGGTACTTTCACAGGGACGAGTCGTCGATATCGAT  >  NC_000913/182503‑182654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑