Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,219	T→C	Y126H (TAC→CAC)	purR →	DNA‑binding transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,219	0	T	C	100.0%	58.0 / NA	18	Y126H (TAC→CAC)	purR	DNA‑binding transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/12);  total (6/12)

AATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACT  >  NC_000913/1738141‑1738282                                                                               |                                                                aaTCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCAc                                                               >  2:282109/1‑81 (MQ=255) aaTCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCAc                                                               >  1:282103/1‑81 (MQ=255)       gcgaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCag                                                           <  2:282076/77‑1 (MQ=255)       gagaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCag                                                           <  2:282075/79‑1 (MQ=255)        agaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCagag                                                         <  1:282077/80‑1 (MQ=255)         gaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCAGAGcc                                                       <  1:282078/81‑1 (MQ=255)               gCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCAGAGCCGTTGCt                                                 >  2:282116/1‑81 (MQ=255)                cGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCAGAGCCGTTGCTg                                                <  2:282079/81‑1 (MQ=255)                 gggCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCAGAGCCGTTGCTgg                                               <  1:282080/81‑1 (MQ=255)                                tgatgGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGt                                <  1:282081/81‑1 (MQ=255)                                  atgGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTa                               <  2:282082/80‑1 (MQ=255)                                   tgGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATc                             <  1:282083/81‑1 (MQ=255)                                        cAAAAACTCGTCGATGGTCTGCTGGTGATGTGTTCTGAGCACCCATAGCCGTTGCTTCCGATGCTGGAAGAGTATCGCCat                        >  2:282113/1‑81 (MQ=255)                                                    gATGGTCTGCTGGTGATGTGTTCTGAGCACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAAtggtg            >  1:282115/1‑81 (MQ=255)                                                        gTCAGCTGGTGATGTGTTCTGAGCACCCAGGGCCGTTGCTGGCGATGCTGAAAGAGTATCGCCATATCCCAATGGTGGTc         <  2:282084/80‑1 (MQ=255)                                                          ctgctgGTGATGTGTTCTGAGCACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATg      >  1:282118/1‑81 (MQ=255)                                                             ctgGTGATGTGTTCTGAGCACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATgg     <  2:282085/79‑1 (MQ=255)                                                                gTGATGTGTTCTGAGCACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACt  <  1:282086/79‑1 (MQ=255)                                                                               |                                                                AATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACT  >  NC_000913/1738141‑1738282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑