Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,750,974	T→A	T14S (ACA→TCA)	ydhX ←	putative 4Fe‑4S ferredoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,750,974	0	T	A	100.0%	88.6 / NA	25	T14S (ACA→TCA)	ydhX	putative 4Fe‑4S ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/13);  total (12/13)

CATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACC  >  NC_000913/1750897‑1751051                                                                              |                                                                              cATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATcc                                                                            >  1:283640/1‑81 (MQ=255)   tGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCa                                                                          >  1:283635/1‑81 (MQ=255)     gCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATc                                                                        <  2:283608/81‑1 (MQ=255)       aTACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGatcccatccc                                                                      <  1:283609/81‑1 (MQ=255)       aTACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGatcccatccc                                                                      >  2:283645/1‑81 (MQ=255)               cTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACaa                                                              <  2:283610/81‑1 (MQ=255)               cTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACa                                                               >  1:283631/1‑80 (MQ=255)                            ccTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGt                                                 <  1:283611/81‑1 (MQ=255)                                gtgtTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGa                                               <  1:283612/79‑1 (MQ=255)                                gtgtTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAg                                             <  1:283613/81‑1 (MQ=255)                                   ttCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGAc                                          <  1:283614/81‑1 (MQ=255)                                     cGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACa                                         <  1:283615/80‑1 (MQ=255)                                          aCAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTg                                     <  2:283616/79‑1 (MQ=255)                                           cAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGAtt                                  <  1:283617/81‑1 (MQ=255)                                             gagaAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTac                                >  2:283637/1‑80 (MQ=255)                                               gaagaCGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATcc                              <  2:283618/81‑1 (MQ=255)                                                   aCGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCt                           >  2:283636/1‑80 (MQ=255)                                                       aGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATg                       >  1:283648/1‑80 (MQ=255)                                                        gAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATg                       >  1:283644/1‑79 (MQ=255)                                                          aCCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGt                   <  2:283619/81‑1 (MQ=255)                                                           ccAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTg                  >  1:283646/1‑81 (MQ=255)                                                           ccAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTg                  >  2:283634/1‑81 (MQ=255)                                                             aGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGc                >  1:283642/1‑81 (MQ=255)                                                              gTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCt               >  1:283655/1‑81 (MQ=255)                                                                       aTTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTc      <  1:283620/81‑1 (MQ=255)                                                                          aCTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCAc   >  1:283649/1‑81 (MQ=255)                                                                           cTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCAcc  <  2:283621/81‑1 (MQ=255)                                                                              |                                                                              CATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACC  >  NC_000913/1750897‑1751051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑