Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,051	T→C	S118S (TCT→TCC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,051	0	T	C	100.0%	100.4 / NA	29	S118S (TCT→TCC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/13);  total (16/13)

TTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAG  >  NC_000913/1755974‑1756121                                                                              |                                                                       ttCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGtt                                                                     >  1:284215/1‑81 (MQ=255)          gATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACAcc                                                            >  2:284213/1‑81 (MQ=255)           aTAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACAcc                                                            <  2:284190/80‑1 (MQ=255)              aaTCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTa                                                         <  2:284191/80‑1 (MQ=255)                  tGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGt                                                    >  2:284234/1‑81 (MQ=255)                    ttATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGtt                                                   >  1:284235/1‑80 (MQ=255)                    ttATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTg                                                  <  2:284192/81‑1 (MQ=255)                         ggCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGAt                                             >  1:284217/1‑81 (MQ=255)                           cAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATgg                                           >  2:284219/1‑81 (MQ=255)                            aaCAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGt                                          >  1:284221/1‑81 (MQ=255)                             aCAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTc                                         <  1:284193/81‑1 (MQ=255)                              cAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCt                                        <  1:284194/81‑1 (MQ=255)                                       ggTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTAt                               >  1:284233/1‑81 (MQ=255)                                       ggTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTAt                               >  1:284226/1‑81 (MQ=255)                                        gTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATg                              >  2:284227/1‑81 (MQ=255)                                         ttGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATgg                             <  2:284195/81‑1 (MQ=255)                                              gTAACGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGt                         >  1:284225/1‑80 (MQ=255)                                                aaCGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTAc                      >  1:284232/1‑81 (MQ=255)                                                  cGTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCg                    <  2:284196/81‑1 (MQ=255)                                                   gTATGAAGGTTTCACTACTTACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGc                   <  2:284197/81‑1 (MQ=255)                                                   gTATGAAGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGc                   >  2:284224/1‑81 (MQ=255)                                                        aaGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCAtt               <  1:284199/80‑1 (MQ=255)                                                        aaGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCAtt               <  1:284198/80‑1 (MQ=255)                                                         aGGTTTCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGa             <  1:284200/81‑1 (MQ=255)                                                            tttCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAgg          >  1:284245/1‑81 (MQ=255)                                                             ttCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAgg          >  2:284229/1‑80 (MQ=255)                                                             ttCACTACTGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAgg          >  2:284241/1‑80 (MQ=255)                                                                    ctGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACaa    <  1:284202/79‑1 (MQ=255)                                                                    ctGACCTGTCCGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAg  <  1:284201/81‑1 (MQ=255)                                                                              |                                                                       TTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAG  >  NC_000913/1755974‑1756121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑