Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	191,485	A→G	E210G (GAA→GGA)	tsf →	protein chain elongation factor EF‑Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	191,485	0	A	G	100.0%	91.3 / NA	27	E210G (GAA→GGA)	tsf	protein chain elongation factor EF‑Ts
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/19);  total (8/19)

GACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTA  >  NC_000913/191409‑191562                                                                             |                                                                              gACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATc                                                                           <  1:41344/81‑1 (MQ=255)  aCGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCg                                                                          >  1:41390/1‑81 (MQ=255)   cGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGc                                                                         <  2:41345/81‑1 (MQ=255)    gTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCa                                                                        <  1:41346/81‑1 (MQ=255)     tATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCag                                                                       <  2:41347/81‑1 (MQ=255)         cGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGaa                                                                   <  1:41348/81‑1 (MQ=255)          gCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCTCAGAGaaa                                                                  <  2:41349/81‑1 (MQ=255)               aGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGt                                                             >  1:41398/1‑81 (MQ=255)                gTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGtt                                                            <  1:41350/81‑1 (MQ=255)                     aGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAgg                                                       >  2:41397/1‑81 (MQ=255)                      gAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGc                                                      <  2:41351/81‑1 (MQ=255)                       aaaaaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGcc                                                     <  2:41352/81‑1 (MQ=255)                        aaaaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGGCg                                                    >  2:41391/1‑81 (MQ=255)                         aaaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCg                                                    >  1:41469/1‑80 (MQ=255)                         aaaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGc                                                   <  1:41353/81‑1 (MQ=255)                           aGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCAt                                                 <  2:41354/81‑1 (MQ=255)                                 ccAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATgaagaa                                           <  1:41355/81‑1 (MQ=255)                                         aGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCg                                   >  2:41402/1‑81 (MQ=255)                                          gCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCgg                                  >  1:41394/1‑81 (MQ=255)                                              gATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGaa                              <  1:41356/81‑1 (MQ=255)                                                  tCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGtt                           <  2:41357/80‑1 (MQ=255)                                                          cAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGAcc                  <  1:41358/81‑1 (MQ=255)                                                          cAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGAcc                  <  2:41359/81‑1 (MQ=255)                                                           aGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCg                 >  1:41396/1‑81 (MQ=255)                                                                 gTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAg            <  1:41360/80‑1 (MQ=255)                                                                    aGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgt        <  2:41361/81‑1 (MQ=255)                                                                      ccGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttc      <  1:41362/81‑1 (MQ=255)                                                                          aaggaaTCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTa  <  1:41363/81‑1 (MQ=255)                                                                             |                                                                              GACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTA  >  NC_000913/191409‑191562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑