Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,136	G→A	A184T (GCC→ACC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,136	0	G	A	100.0%	77.3 / NA	22	A184T (GCC→ACC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/9);  total (13/9)

CTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATT  >  NC_000913/1776059‑1776212                                                                              |                                                                             cTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCt                                                                           <  2:286680/81‑1 (MQ=255)    aagaagACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCa                                                                        >  1:286716/1‑81 (MQ=255)          aCCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTcgcg                                                                  >  2:286713/1‑81 (MQ=255)           ccTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCgcgc                                                                 >  1:286717/1‑81 (MQ=255)               aTTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTacc                                                             <  1:286681/81‑1 (MQ=255)                          cGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAg                                                  >  2:286722/1‑81 (MQ=255)                           gAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGt                                                 >  2:286710/1‑81 (MQ=255)                             gTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGa                                               <  1:286682/81‑1 (MQ=255)                               ttGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAg                                             >  1:286711/1‑81 (MQ=255)                                 gATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAgg                                            <  1:286683/80‑1 (MQ=255)                                 gATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGc                                           >  1:286704/1‑81 (MQ=255)                                  aTAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCt                                          <  2:286684/81‑1 (MQ=255)                                       aaaGAATATCTCTATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGc                                      >  2:286712/1‑80 (MQ=255)                                                ctctATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTg                              >  2:286714/1‑79 (MQ=255)                                                  ctATTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTg                              >  2:286718/1‑77 (MQ=255)                                                    aTTACAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGAt                         >  2:286715/1‑80 (MQ=255)                                                       aCAAAGCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAg                      <  1:286685/80‑1 (MQ=255)                                                            gCGATTGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATg                >  1:286734/1‑81 (MQ=255)                                                                 tGCGCCAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTAtct           >  2:286727/1‑81 (MQ=255)                                                                     ccAGATATTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGAc       <  1:286686/81‑1 (MQ=255)                                                                       aGATATTACCTTCATTCGCGCTACCACCTGAGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGc     <  1:286687/81‑1 (MQ=255)                                                                          tatTACCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCAtt  <  2:286688/81‑1 (MQ=255)                                                                              |                                                                             CTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATT  >  NC_000913/1776059‑1776212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑