Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,781,876	G→A	G161D (GGC→GAC)	ydiS →	putative oxidoreductase with FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,781,876	0	G	A	100.0%	72.7 / NA	21	G161D (GGC→GAC)	ydiS	putative oxidoreductase with FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/11);  total (10/11)

TGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTA  >  NC_000913/1781802‑1781950                                                                           |                                                                           tGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTaa                                                                      >  1:287424/1‑81 (MQ=255)    aGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTc                                                                   <  1:287382/81‑1 (MQ=255)    aGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTc                                                                   <  2:287381/81‑1 (MQ=255)     ggAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCg                                                                  <  2:287383/81‑1 (MQ=255)               tCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACGCGATGCTTGGcc                                                        <  2:287384/81‑1 (MQ=255)                    ggCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCcgctcg                                                   >  2:287415/1‑81 (MQ=255)                       gTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTg                                                >  1:287410/1‑81 (MQ=255)                                ggggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGtt                                       >  1:287412/1‑81 (MQ=255)                                 gggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGtt                                       <  1:287386/80‑1 (MQ=255)                                 gggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTc                                      <  2:287385/81‑1 (MQ=255)                                  ggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc                                     <  2:287387/81‑1 (MQ=255)                                    atgatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCg                                   <  2:287388/81‑1 (MQ=255)                                      gatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCt                                 >  2:287416/1‑81 (MQ=255)                                        tatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTc                               >  1:287429/1‑81 (MQ=255)                                         atTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTcc                              >  1:287408/1‑81 (MQ=255)                                          ttCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTcc                              <  2:287389/80‑1 (MQ=255)                                                  gCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGcat                     <  2:287390/81‑1 (MQ=255)                                                       tgtgGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTa                >  1:287420/1‑81 (MQ=255)                                                         tggtgATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACg              >  1:287428/1‑81 (MQ=255)                                                               ttCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTg        <  1:287391/81‑1 (MQ=255)                                                                       gATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTa  >  2:287431/1‑79 (MQ=255)                                                                           |                                                                           TGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTA  >  NC_000913/1781802‑1781950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑