Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,785,652	C→T	M487I (ATG→ATA)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,785,652	0	C	T	100.0%	68.7 / NA	20	M487I (ATG→ATA)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/10);  total (10/10)

CCAGACGCGCAAGGCCCACGCCTTCGTTCGGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATA  >  NC_000913/1785576‑1785726                                                                             |                                                                           ccAGACGCGCAAGGCCCACGCCTTCGTTCGGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTtcattatca                                                                        >  2:287891/1‑81 (MQ=255)    gACGCGCAAGGCCCACGCCTTCGTTCGGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACtt                                                                     <  2:287867/81‑1 (MQ=255)    gACGCGCAAGGCCCACGCCTTCGTTCGGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACtt                                                                     <  1:287866/81‑1 (MQ=255)    gACGCGCAAGGCCCACGCCTTCGTTCGGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACtt                                                                     >  2:287888/1‑81 (MQ=255)       gcgcAAGGCCCACGCCTTCGTTCGGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTc                                                                   >  2:287893/1‑80 (MQ=255)       gcgcAAGGCCCACGCCTTCGTTCGGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTc                                                                   >  2:287885/1‑80 (MQ=255)          cAAGGCCCACGCCTTCGTTCGGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACg                                                               <  1:287868/81‑1 (MQ=255)                         cgttcgGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATc                                                 <  1:287869/80‑1 (MQ=255)                          gttcgGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGt                                               >  2:287898/1‑81 (MQ=255)                             cgGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTc                                            >  2:287897/1‑81 (MQ=255)                                  ggcaggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACg                                        <  1:287870/80‑1 (MQ=255)                                   gcaggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCt                                      >  1:287900/1‑81 (MQ=255)                                     aggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTgg                                    <  2:287871/81‑1 (MQ=255)                                       gcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAg                                  >  1:287899/1‑81 (MQ=255)                                          aaGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCtt                               <  2:287872/81‑1 (MQ=255)                                          aaGTCGAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCtt                               >  2:287905/1‑81 (MQ=255)                                               gAAAGCACGGTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCAc                          <  2:287873/81‑1 (MQ=255)                                                       ggTCCGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAAtt                  >  1:287890/1‑81 (MQ=255)                                                           cGGGTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCa               <  1:287874/80‑1 (MQ=255)                                                              gTTACCGACGTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCaa           >  2:287894/1‑81 (MQ=255)                                                                       gTTCATTATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATa  <  1:287875/81‑1 (MQ=255)                                                                             |                                                                           CCAGACGCGCAAGGCCCACGCCTTCGTTCGGTAGGCAGGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATA  >  NC_000913/1785576‑1785726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑