Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	192,324	A→G	E157G (GAA→GGA)	pyrH →	UMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	192,324	0	A	G	100.0%	106.5 / NA	32	E157G (GAA→GGA)	pyrH	UMP kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/19);  total (13/19)

GTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGC  >  NC_000913/192248‑192396                                                                             |                                                                         gtgATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGcc                                                                      <  1:41513/81‑1 (MQ=255)   gATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGa                                                                    >  1:41551/1‑81 (MQ=255)    aTCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGAt                                                                   >  1:41547/1‑81 (MQ=255)     tCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGAtg                                                                  >  1:41567/1‑81 (MQ=255)      ccTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGAtgt                                                                 >  1:41561/1‑81 (MQ=255)         ctcCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATgtggt                                                              >  1:41550/1‑81 (MQ=255)            cgccgGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGc                                                            <  1:41514/80‑1 (MQ=255)             gccgGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCt                                                           >  2:41566/1‑80 (MQ=255)                ggTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGaaa                                                       <  1:41515/81‑1 (MQ=255)                        tAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCaa                                               <  1:41516/81‑1 (MQ=255)                               ttCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGAc                                        <  2:41517/81‑1 (MQ=255)                               ttCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGAc                                        <  1:41518/81‑1 (MQ=255)                                tCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTTACg                                       >  1:41621/1‑81 (MQ=255)                                    tACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACgg                                      <  1:41519/78‑1 (MQ=255)                                     accaccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCgt                                   <  2:41520/80‑1 (MQ=255)                                      ccaccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCgtgt                                 >  2:41565/1‑81 (MQ=255)                                       caccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGtt                                >  2:41564/1‑81 (MQ=255)                                       caccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGtt                                <  2:41521/81‑1 (MQ=255)                                        accGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGttt                               <  2:41522/81‑1 (MQ=255)                                         ccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTa                              <  2:41524/81‑1 (MQ=255)                                         ccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTa                              <  1:41523/81‑1 (MQ=255)                                           gACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTAcc                            >  1:41570/1‑81 (MQ=255)                                            aCTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCg                           <  2:41525/81‑1 (MQ=255)                                                   agcTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATcc                    >  1:41556/1‑81 (MQ=255)                                                    gcTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCg                   <  2:41526/81‑1 (MQ=255)                                                    gcTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCg                   <  1:41527/81‑1 (MQ=255)                                                        gCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGc                 <  1:41528/79‑1 (MQ=255)                                                        gCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCg                >  1:41573/1‑80 (MQ=255)                                                               tGGTATCGAAATTGGAGCCGATGTGGTGCTGGAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATcc        <  2:41529/81‑1 (MQ=255)                                                                  tATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAAc     >  1:41568/1‑81 (MQ=255)                                                                    tCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCg   <  2:41530/81‑1 (MQ=255)                                                                      gAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGc  <  2:41531/80‑1 (MQ=255)                                                                             |                                                                         GTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGC  >  NC_000913/192248‑192396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑