Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,787,109	A→G	S2P (TCC→CCC)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,787,109	0	A	G	100.0%	37.5 / NA	12	S2P (TCC→CCC)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/8);  total (4/8)

TTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACC  >  NC_000913/1787031‑1787185                                                                               |                                                                             ttttGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCATAGCACCAGCGGTGACGAGCCATTGTTGGGCa                                                                            >  2:288104/1‑81 (MQ=255)          cAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAAt                                                                   <  2:288081/81‑1 (MQ=255)             cccTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCt                                                                <  1:288082/81‑1 (MQ=255)                 gTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTtg                                                            >  2:288103/1‑81 (MQ=255)                  tCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTtg                                                            <  2:288083/80‑1 (MQ=255)                             catGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGaa                                                 <  1:288084/80‑1 (MQ=255)                             catGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAAc                                                >  1:288100/1‑81 (MQ=255)                                           tataCCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAaca                                   >  1:288101/1‑80 (MQ=255)                                                       ccAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCAt                      <  2:288085/81‑1 (MQ=255)                                                        cAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCAtt                     <  2:288086/81‑1 (MQ=255)                                                         aGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCAttt                    <  1:288087/81‑1 (MQ=255)                                                                           ttgGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAAcc  <  1:288088/81‑1 (MQ=255)                                                                               |                                                                             TTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACC  >  NC_000913/1787031‑1787185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑